Difference between revisions of "Part:BBa K566002"
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The biphasic switch combines positive and negative regulation through a single input. It is turned on by low lambda cI concentrations and off by high cI concentrations.
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The biphasic switch combines positive and negative regulation through a single input. It is turned on by low lambda cI concentrations and off by high cI concentrations<a href="https://parts.igem.org/Part:BBa_K566002:Design" class="references-link">[1]</a>. There are two well-known sets of cI binding sites in lambda (OR and OL) spaced 2.4 kb apart and composed of three operators each (OR1:OR2:OR3; OL1:OL2:OL3). cI has a higher affinity for OR1 and OR2 operators, where binding positively regulates the pRM promoter. In presence of the OL set, cI octamerise specifically binding to the two sets of operators (OR and OL) at the same time and therefore forming a DNA loop[2]. Such structure stabilizes cI's binding to OR3, allowing pRM's repression at high concentrations.  
 
   
 
   
 
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Revision as of 16:23, 27 September 2011

Biphasic switch


The biphasic switch combines positive and negative regulation through a single input. It is turned on by low lambda cI concentrations and off by high cI concentrations<a href="https://parts.igem.org/Part:BBa_K566002:Design" class="references-link">[1]</a>. There are two well-known sets of cI binding sites in lambda (OR and OL) spaced 2.4 kb apart and composed of three operators each (OR1:OR2:OR3; OL1:OL2:OL3). cI has a higher affinity for OR1 and OR2 operators, where binding positively regulates the pRM promoter. In presence of the OL set, cI octamerise specifically binding to the two sets of operators (OR and OL) at the same time and therefore forming a DNA loop[2]. Such structure stabilizes cI's binding to OR3, allowing pRM's repression at high concentrations.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 95
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]