Difference between revisions of "Part:BBa K678049"

Latest revision as of 15:45, 27 September 2011

pJEJAM1

pJEJAM1 is a plasmid intended for transient transfection of mammalian cells. pJEJAM1 consists of the strong constitutive cytomegalovirus (CMV) promoter, green fluorescence protein (GFP), the BGH polyA, the hygR marker cassette, an ori for propagation in E.coli, and an ampR resistance cassette (Figure 1).

DTU-Denmark-2 2011 Figure 1: Plasmid map of pJEJAM1, the figure is not drawn to scale.

Expression of pJEJAM1 in the mammalian cell line U-2 OS results in a homogenous distribution of GFP in the cells (Figure 2 and Figure 3).

DTU-Denmark-2 2011 Figure 2: U-2 OS cells transiently transfected with pJEJAM1 expressing GFP. The white bar has a length of 20μm.

DTU-Denmark-2 2011 Figure 3: U-2 OS cells transiently transfected with pJEJAM1 expressing GFP. The white bar has a length of 20μm.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 2216
Illegal PstI site found at 2306
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 2216
Illegal PstI site found at 2306
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 2216
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 2216
Illegal PstI site found at 2306
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 2216
Illegal PstI site found at 2306
Illegal NgoMIV site found at 3071
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K678049 short</partinfo>
-pJEJAM1 is a plasmid intended for transient transfection of mammalian cells. pJEJAM1 consists of the strong constitutive cytomegalovirus (CMV) promoter, green fluorescence protein (GFP), the ''BGK'' polyA, the ''hygR'' marker cassette used for selection, an ori for propagation in ''E.coli'' and and ampR resistance cassette (Figure 1).
+pJEJAM1 is a plasmid intended for transient transfection of mammalian cells. pJEJAM1 consists of the strong constitutive cytomegalovirus (CMV) promoter, green fluorescence protein (GFP), the BGH polyA, the ''hygR'' marker cassette, an ori for propagation in ''E.coli'', and an ampR resistance cassette (Figure 1).
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 <br>
-[[Image:Devices_13.png|none|500px|thumb|<b>DTU-Denmark-2 2011</b> Figure 1: Plasmid map of pJEJAM15, the figure is not drawn to scale.]]
+[[Image:Devices_13.png|none|500px|thumb|<b>DTU-Denmark-2 2011</b> Figure 1: Plasmid map of pJEJAM1, the figure is not drawn to scale.]]
 <br>
 <br>
-consists of RFP with a target signal to the nucleus which can explain the spots compared to the results from device and the spores with a single nuclei contains a lot of RFP signal. We cannot conclude that the signal is accumulated in the nucleus since they are not dyed, though can it be concluded that the RFP signal is target a specific place and accumulated somewhere.
+Expression of pJEJAM1 in the mammalian cell line U-2 OS results in a homogenous distribution of GFP in the cells (Figure 2 and Figure 3).
-[[Image:IGEM_001.png|left|300px|thumb|<b>DTU-Denmark-2 2011</b> Figure 2: .]]
-[[Image:IGEM3.png|right|300px|thumb|<b>DTU-Denmark-2 2011</b> Figure 3: .]]
+[[Image:IGEM_001.png|left|425px|thumb|<b>DTU-Denmark-2 2011</b> Figure 2: U-2 OS cells transiently transfected with pJEJAM1 expressing GFP. The white bar has a length of 20μm.]]
+[[Image:IGEM3.png|right|425px|thumb|<b>DTU-Denmark-2 2011</b> Figure 3: U-2 OS cells transiently transfected with pJEJAM1 expressing GFP. The white bar has a length of 20μm.]]
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