Difference between revisions of "Part:BBa K524100:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K524100 short</partinfo>
 
<partinfo>BBa_K524100 short</partinfo>
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===Source===
 
===Source===
  
Amplified from genomic DNA of Escherichia coli K-12 Strain RR1 via PCR
+
Amplified from genomic DNA of Escherichia coli K-12 Strain RR1
  
 
===References===
 
===References===

Revision as of 13:22, 27 September 2011

Bcr - multidrug efflux system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 503
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 99
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

'GTG' start codon has been mutated to 'ATG'. Stop codons at the end of the coding sequence have been changed to 'TAATAA'.


Source

Amplified from genomic DNA of Escherichia coli K-12 Strain RR1

References