Difference between revisions of "Part:BBa K678002:Design"

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This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains the linkers as shown on the figure below.
 
This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains the linkers as shown on the figure below.
  
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https://static.igem.org/mediawiki/igem.org/2/26/Overveiw_module_tailst2.png
 
  
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This part was USER cloned into the shipping plasmid pSB1C3 but is also contained in the Plug'n'Play plasmid pJEJAM2
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[[Image:Overveiw_module_tails_2.png|center|450px|caption|<b>DTU-Denmark-2 2011</b>]]
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This part was USER cloned into the shipping plasmid pSB1C3 but is also contained in the Plug'n'Play plasmid [https://parts.igem.org/wiki/index.php?title=Part:BBa_K678050 pJEJAM2]
  
 
===Source===
 
===Source===
  
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Source to be announced
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[https://parts.igem.org/wiki/index.php?title=Part:BBa_J52034 CMV promoter], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K678019 BGH poly A], and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K678021 Hygromycin cassette]
  
 
===References===
 
===References===

Latest revision as of 09:55, 27 September 2011

eGFP-PTS1 for mammalian cells


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2233
    Illegal SpeI site found at 49
    Illegal PstI site found at 2323
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2233
    Illegal SpeI site found at 49
    Illegal PstI site found at 2323
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2233
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2233
    Illegal SpeI site found at 49
    Illegal PstI site found at 2323
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2233
    Illegal SpeI site found at 49
    Illegal PstI site found at 2323
    Illegal NgoMIV site found at 3088
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains the linkers as shown on the figure below.


DTU-Denmark-2 2011

This part was USER cloned into the shipping plasmid pSB1C3 but is also contained in the Plug'n'Play plasmid pJEJAM2

Source

CMV promoter, BGH poly A, and Hygromycin cassette

References