Difference between revisions of "Part:BBa K678002:Design"

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===Design Notes===
 
===Design Notes===
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This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains the linker 1 at the 5'-end and the linker 6 at the 3'-end as shown on the figure below.
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This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains the linkers as shown on the figure below.
  
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https://static.igem.org/mediawiki/igem.org/2/26/Overveiw_module_tailst2.png
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[[Image:Overveiw_module_tails_2.png|center|450px|caption|<b>DTU-Denmark-2 2011</b>]]
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This part was USER cloned into the shipping plasmid pSB1C3 but is also contained in the Plug'n'Play plasmid [https://parts.igem.org/wiki/index.php?title=Part:BBa_K678050 pJEJAM2]
  
 
===Source===
 
===Source===
  
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Source to be announced
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[https://parts.igem.org/wiki/index.php?title=Part:BBa_J52034 CMV promoter], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K678019 BGH poly A], and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K678021 Hygromycin cassette]
  
 
===References===
 
===References===

Latest revision as of 09:55, 27 September 2011

eGFP-PTS1 for mammalian cells


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2233
    Illegal SpeI site found at 49
    Illegal PstI site found at 2323
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2233
    Illegal SpeI site found at 49
    Illegal PstI site found at 2323
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2233
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2233
    Illegal SpeI site found at 49
    Illegal PstI site found at 2323
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2233
    Illegal SpeI site found at 49
    Illegal PstI site found at 2323
    Illegal NgoMIV site found at 3088
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains the linkers as shown on the figure below.


DTU-Denmark-2 2011

This part was USER cloned into the shipping plasmid pSB1C3 but is also contained in the Plug'n'Play plasmid pJEJAM2

Source

CMV promoter, BGH poly A, and Hygromycin cassette

References