Difference between revisions of "Part:BBa K123003:Experience"
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We ran the DNA sequence through the ExPASy translator with the following result: | We ran the DNA sequence through the ExPASy translator with the following result: | ||
| − | MAGMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAA | + | MAGMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAA |
| − | YEFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSP | + | YEFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSP |
| − | FLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESA | + | FLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESA |
| − | KETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQA | + | KETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQA |
| − | CRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEVGSTG-RKRRNTRWPATCVR | + | CRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEVGSTG-RKRRNTRWPATCVR |
| − | RTCGRLR--SNVLKKTLWRCL-PRTRWFLRCWTRNRRSCTLNTTRPVRSLKRL-WVC-PT | + | RTCGRLR--SNVLKKTLWRCL-PRTRWFLRCWTRNRRSCTLNTTRPVRSLKRL-WVC-PT |
| − | WRTVNWFT-STGRNVFRVSLT-PCTTRFTCWNARGWKS--SVWFGVLWNIRVNCCSRRTC | + | WRTVNWFT-STGRNVFRVSLT-PCTTRFTCWNARGWKS--SVWFGVLWNIRVNCCSRRTC |
| − | CWTVTRVNALKVWLKSSTCCWRPLLVSV--TYKVKSLFA-NLSSC-TLVFTPSCLLP-NL | + | CWTVTRVNALKVWLKSSTCCWRPLLVSV--TYKVKSLFA-NLSSC-TLVFTPSCLLP-NL |
| − | WKKKTTSTVFWTKSPTP-ST-WRKRV-PYNSSTSVWRSCC-SCLTSVTCLTKVWNTCTL- | + | WKKKTTSTVFWTKSPTP-ST-WRKRV-PYNSSTSVWRSCC-SCLTSVTCLTKVWNTCTL- |
| − | NAKTLFRCTTCCWKCWTRTVCTRRPLVVVRLLKKPTSLTWRPRVLPLLTLYKNTTSPAKR | + | NAKTLFRCTTCCWKCWTRTVCTRRPLVVVRLLKKPTSLTWRPRVLPLLTLYKNTTSPAKR |
| − | KVSRRPLPV | + | KVSRRPLPV |
It looks like there's an intron in the coding sequence. This should be the correct sequence: | It looks like there's an intron in the coding sequence. This should be the correct sequence: | ||
>gi|306921463|dbj|BAJ17811.1| estrogen receptor 1 [synthetic construct] | >gi|306921463|dbj|BAJ17811.1| estrogen receptor 1 [synthetic construct] | ||
| − | MTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAYEFNAAAAANA | + | MTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAYEFNAAAAANA |
| − | QVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYT | + | QVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYT |
| − | VREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAKETRYCAVCNDYASGYHYGVWSCEGCKAFFK | + | VREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAKETRYCAVCNDYASGYHYGVWSCEGCKAFFK |
| − | RSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEV | + | RSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEV |
| − | GSAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNLA | + | GSAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNLA |
| − | DRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEG | + | DRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEG |
| − | MVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLM | + | MVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLM |
| − | AKAGLTLQQQHQRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASV | + | AKAGLTLQQQHQRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASV |
| − | EETDQSHLATAGSTSSHSLQKYYITGEAEGFPATV | + | EETDQSHLATAGSTSSHSLQKYYITGEAEGFPATV |
| + | Update: September 27, 2011: We sequenced most of the gene. It seems the team codon optimized and submitted a different sequence in silico than what the physical DNA actually is. Here is the sequence: | ||
| + | ATGACTCTGCACACTAAAGCATCTGGCATGGCGCTGCTGCATCAGATCCA | ||
| + | GGGCAACGAACTGGAGCCGCTGAATCGCCCGCAGCTGAAGATCCCACTGG | ||
| + | AACGTCCGCTGGGCGAGGTCTACCTGGATTCCAGCAAGCCAGCTGTATAT | ||
| + | AACTATCCGGAAGGTGCAGCGTACGAATTTAACGCTGCGGCTGCGGCCAA | ||
| + | CGCCCAAGTTTACGGCCAAACTGGTCTGCCGTATGGCCCTGGTAGCGAAG | ||
| + | CAGCCGCTTTCGGTAGCAACGGCCTGGGTGGTTTTCCGCCTCTGAACTCT | ||
| + | GTATCTCCGTCCCCGCTGATGCTGCTGCACCCTCCGCCGCAGCTGTCTCC | ||
| + | GTTCCTGCAACCGCACGGCCAGCAGGTGCCGTACTACCTGNNNNNNNNNN | ||
| + | NNNNNNNNNNNNNNGTGCGTGAGGCTGGTCCACCGGCGTTCTATCGTCCG | ||
| + | AATAGCGACAACCGTCGCCAGGGCGGTCGTGAACGCCTGGCTTCCACCAA | ||
| + | CGACAAAGGCTCTATGGCTATGGAATCTGCGAAAGAAACCCGTTACTGTG | ||
| + | CAGTGTGTAACGACTATGCATCCGGTTACCATTACGGCGTTTGGAGCTGT | ||
| + | GAGGGCTGCAAAGCGTTCTTCAAGCGCTCCATTCAGGGTCACAACGATTA | ||
| + | CATGTGCCCGGCCACCAATCAGTGCACGATCGACAAAAACCGTCGTAAAT | ||
| + | CCTGCCAGGCATGTCGTCTGCGTAAATGCTATGAAGTTGGCATGATGAAA | ||
| + | GGTGGTATTCGTAAAGATCGCCGCGGTGGCCGTATGCTGAAACACAAACG | ||
| + | TCAGCGCGATGACGGTGAAGGTCGTGGTGAAGTTGGTTCTACCGGCGATA | ||
| + | TGCGCGCAGCTAATCTGTGGCCTTCCCCACTGATGATCAAACGTTCTAAG | ||
| + | AAAAACAGCCTGGCGCTGTCCCTGACCGCCGACCAGATGGTATCCGCACT | ||
| + | GCTGGACGCAGAACCGCCGATTCTGTACAGCGAGTACGACCCAACGCGCC | ||
| + | CGTTTAGCGAGGCAAGCATGATGGGTCTGCTGACCAACCTGGCTGATCGT | ||
| + | GAACTGGTCCATATGATCAACTGGGCGAAACGCGTTCCGGGTTTCGTAGA | ||
| + | CCTGACTCTGCACGACCAGGTCCACCTGCTGGAATGTGCATGGCTGGAGA | ||
| + | TCCTGATGATCGGCCTGGTTTGGCGTTCCATGGAACACCCGGGTAAGCTG | ||
| + | CTGTTTGCCCCGAACCTGCTGCTGGACCGTAACCAGGGTAANTGCGTGGA | ||
| + | AGGCATGGTTGAAATTTTCGATATGCTGCTGGCCACCTCTAGCCGTTTCC | ||
| + | GCATGATGAACCTGCAAGGTGAAGAGTTCGTGTGCCTGAAATCCATCATT | ||
| + | CTGCTGAACTCCGGCGTGTATACGTTCCTGAGCTCTACCCTGAAATCTCT | ||
| + | GGAGGAAAAAGATCACATCCACCGTGTGCTGGACAAAATCACTGATACCC | ||
| + | TGATTCACCTGATGGCAAAAGCTGGCCTGACCCTGCAACAGCAGCACCAG | ||
| + | CGTCTGGCGCAGCTGCTGCTGATCCTGTCTCACATCCGCCACATGAGCAA | ||
| + | TAAGGGTATGGAACATCTGTACTCTATGAAATGTAAAAACGTTGTTCCGC | ||
| + | TGTACGATCTGCTGCTGGAAATGCTGGACGCCCACCGTCTGCATGCGCCG | ||
| + | ACCAGCCGTGGCGGTGCTTCCGTTGAAGAAACTGATCAGTCTCATCTGGC | ||
| + | GACCGCTGGCTCCACCTCTTCCCACTCTCTGCAAAAATATTACATTACTG | ||
| + | GTGAAGCGGAAGGCTTCCCGGCGACCGTAACCGGTTAA | ||
|}; | |}; | ||
<!-- DON'T DELETE --><partinfo>BBa_K123003 EndReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K123003 EndReviews</partinfo> | ||
Latest revision as of 07:23, 27 September 2011
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UNIQd5536552bd2ced5e-partinfo-00000000-QINU
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jbrown2 ([http://2011.igem.org/Team:Caltech Caltech iGEM team 2011]) |
We ran the DNA sequence through the ExPASy translator with the following result: MAGMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAA YEFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSP FLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESA KETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQA CRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEVGSTG-RKRRNTRWPATCVR RTCGRLR--SNVLKKTLWRCL-PRTRWFLRCWTRNRRSCTLNTTRPVRSLKRL-WVC-PT WRTVNWFT-STGRNVFRVSLT-PCTTRFTCWNARGWKS--SVWFGVLWNIRVNCCSRRTC CWTVTRVNALKVWLKSSTCCWRPLLVSV--TYKVKSLFA-NLSSC-TLVFTPSCLLP-NL WKKKTTSTVFWTKSPTP-ST-WRKRV-PYNSSTSVWRSCC-SCLTSVTCLTKVWNTCTL- NAKTLFRCTTCCWKCWTRTVCTRRPLVVVRLLKKPTSLTWRPRVLPLLTLYKNTTSPAKR KVSRRPLPV It looks like there's an intron in the coding sequence. This should be the correct sequence: >gi|306921463|dbj|BAJ17811.1| estrogen receptor 1 [synthetic construct] MTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAYEFNAAAAANA QVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYT VREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAKETRYCAVCNDYASGYHYGVWSCEGCKAFFK RSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEV GSAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNLA DRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEG MVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLM AKAGLTLQQQHQRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASV EETDQSHLATAGSTSSHSLQKYYITGEAEGFPATV Update: September 27, 2011: We sequenced most of the gene. It seems the team codon optimized and submitted a different sequence in silico than what the physical DNA actually is. Here is the sequence: ATGACTCTGCACACTAAAGCATCTGGCATGGCGCTGCTGCATCAGATCCA GGGCAACGAACTGGAGCCGCTGAATCGCCCGCAGCTGAAGATCCCACTGG AACGTCCGCTGGGCGAGGTCTACCTGGATTCCAGCAAGCCAGCTGTATAT AACTATCCGGAAGGTGCAGCGTACGAATTTAACGCTGCGGCTGCGGCCAA CGCCCAAGTTTACGGCCAAACTGGTCTGCCGTATGGCCCTGGTAGCGAAG CAGCCGCTTTCGGTAGCAACGGCCTGGGTGGTTTTCCGCCTCTGAACTCT GTATCTCCGTCCCCGCTGATGCTGCTGCACCCTCCGCCGCAGCTGTCTCC GTTCCTGCAACCGCACGGCCAGCAGGTGCCGTACTACCTGNNNNNNNNNN NNNNNNNNNNNNNNGTGCGTGAGGCTGGTCCACCGGCGTTCTATCGTCCG AATAGCGACAACCGTCGCCAGGGCGGTCGTGAACGCCTGGCTTCCACCAA CGACAAAGGCTCTATGGCTATGGAATCTGCGAAAGAAACCCGTTACTGTG CAGTGTGTAACGACTATGCATCCGGTTACCATTACGGCGTTTGGAGCTGT GAGGGCTGCAAAGCGTTCTTCAAGCGCTCCATTCAGGGTCACAACGATTA CATGTGCCCGGCCACCAATCAGTGCACGATCGACAAAAACCGTCGTAAAT CCTGCCAGGCATGTCGTCTGCGTAAATGCTATGAAGTTGGCATGATGAAA GGTGGTATTCGTAAAGATCGCCGCGGTGGCCGTATGCTGAAACACAAACG TCAGCGCGATGACGGTGAAGGTCGTGGTGAAGTTGGTTCTACCGGCGATA TGCGCGCAGCTAATCTGTGGCCTTCCCCACTGATGATCAAACGTTCTAAG AAAAACAGCCTGGCGCTGTCCCTGACCGCCGACCAGATGGTATCCGCACT GCTGGACGCAGAACCGCCGATTCTGTACAGCGAGTACGACCCAACGCGCC CGTTTAGCGAGGCAAGCATGATGGGTCTGCTGACCAACCTGGCTGATCGT GAACTGGTCCATATGATCAACTGGGCGAAACGCGTTCCGGGTTTCGTAGA CCTGACTCTGCACGACCAGGTCCACCTGCTGGAATGTGCATGGCTGGAGA TCCTGATGATCGGCCTGGTTTGGCGTTCCATGGAACACCCGGGTAAGCTG CTGTTTGCCCCGAACCTGCTGCTGGACCGTAACCAGGGTAANTGCGTGGA AGGCATGGTTGAAATTTTCGATATGCTGCTGGCCACCTCTAGCCGTTTCC GCATGATGAACCTGCAAGGTGAAGAGTTCGTGTGCCTGAAATCCATCATT CTGCTGAACTCCGGCGTGTATACGTTCCTGAGCTCTACCCTGAAATCTCT GGAGGAAAAAGATCACATCCACCGTGTGCTGGACAAAATCACTGATACCC TGATTCACCTGATGGCAAAAGCTGGCCTGACCCTGCAACAGCAGCACCAG CGTCTGGCGCAGCTGCTGCTGATCCTGTCTCACATCCGCCACATGAGCAA TAAGGGTATGGAACATCTGTACTCTATGAAATGTAAAAACGTTGTTCCGC TGTACGATCTGCTGCTGGAAATGCTGGACGCCCACCGTCTGCATGCGCCG ACCAGCCGTGGCGGTGCTTCCGTTGAAGAAACTGATCAGTCTCATCTGGC GACCGCTGGCTCCACCTCTTCCCACTCTCTGCAAAAATATTACATTACTG GTGAAGCGGAAGGCTTCCCGGCGACCGTAACCGGTTAA |
UNIQd5536552bd2ced5e-partinfo-00000002-QINU