Difference between revisions of "Part:BBa K554002"
Line 2: Line 2:
 
<partinfo>BBa_K554002 short</partinfo>
 
<partinfo>BBa_K554002 short</partinfo>
  
−
HlyA is ...
+
The HlyA is a signal peptide found in the C-terminal signal sequence of alpha-hemolysin (HlyA). It is used to target proteins for secretion via the Type I secretion pathway of gram-negative bacteria. Fusion of the HlyA signal peptide to the target protein may result in transport of the protein from the cytoplasm to the extracellular medium in a single step. HlyA, the signal sequence, seems to interact with the cytoplasmic region of the pre-formed HlyB–D complex. After the binding of the HlyA secretion signal by the HlyB–D complex, HlyD induces the interaction with TolC. To be effective, only the last 50-60 aminoacids of the C-terminal of Hly-A are required, so we can use it as a fusion protein that is attached to the interleukins (such as IL-12 and IL-10) and allows it to be secreted. Besides, HlyA is itself a weak antigen, turning it harmeless to the immune responses we are trying to interfere with.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 16: Line 16:
 
<partinfo>BBa_K554002 parameters</partinfo>
 
<partinfo>BBa_K554002 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
===Usage and Biology===
 +
'''You can see a representation of this device acting in the schema below:''' HlyB gene and product are shown as a symbolic cilinder in orange.
 +
 +
[[Image:UNICAMP_EMSE_secretion_device_schema.jpg|center|700px]]
 +
'''Representation of device 3, the protein secretion system, in a Jedi bacteria that contains Device 1 (Adrenaline sensor/IL-12 producer). To export a protein, the bacteria must have the HlyD, HlyB and TolC proteins and the target protein must have a signal sequence (HlyA tail). In this case, the target protein to be secreted is IL-12.
 +
 +
A more realistic schema of ABC transport system is shown below:
 +
[[Image:UNICAMP_EMSE_secretion_ABC.jpg|center|500px]]

Revision as of 05:26, 27 September 2011

HlyA secretion signal peptide

The HlyA is a signal peptide found in the C-terminal signal sequence of alpha-hemolysin (HlyA). It is used to target proteins for secretion via the Type I secretion pathway of gram-negative bacteria. Fusion of the HlyA signal peptide to the target protein may result in transport of the protein from the cytoplasm to the extracellular medium in a single step. HlyA, the signal sequence, seems to interact with the cytoplasmic region of the pre-formed HlyB–D complex. After the binding of the HlyA secretion signal by the HlyB–D complex, HlyD induces the interaction with TolC. To be effective, only the last 50-60 aminoacids of the C-terminal of Hly-A are required, so we can use it as a fusion protein that is attached to the interleukins (such as IL-12 and IL-10) and allows it to be secreted. Besides, HlyA is itself a weak antigen, turning it harmeless to the immune responses we are trying to interfere with.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

You can see a representation of this device acting in the schema below: HlyB gene and product are shown as a symbolic cilinder in orange.

UNICAMP EMSE secretion device schema.jpg

Representation of device 3, the protein secretion system, in a Jedi bacteria that contains Device 1 (Adrenaline sensor/IL-12 producer). To export a protein, the bacteria must have the HlyD, HlyB and TolC proteins and the target protein must have a signal sequence (HlyA tail). In this case, the target protein to be secreted is IL-12.

A more realistic schema of ABC transport system is shown below:

UNICAMP EMSE secretion ABC.jpg