Difference between revisions of "Part:BBa K530000"
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[[Image:CrtYB Plasmid Map.png]]
 
[[Image:CrtYB Plasmid Map.png]]
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===Sequencing===
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[[Image:CrtYB Sequencing.png]]
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This is the sequencing for colony 2. Colony 2 had areas of poor match between both forward and reverse regions. We attribute this to the fact that our gene is far too big for two primers to cover on their own. The middle regions are due to this, and the initial and final regions are due to the trace taking some time to start up.

Revision as of 01:51, 27 September 2011

CRTYB

Enzyme in the pathway required for B-Carotene Synthesis. This enzyme is a combination, it is the full Phytoene Synthase enzyme spliced with a Lycopene B-Cyclase enzymatic domain. This sequence was taken from a WT strain of xanthophyllomyces dendrorhous. It catalyzes both the conversion of Geranylgeranyl diphosphate to Phytoene and Lycopene to B-Carotene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1068
    Illegal BsaI.rc site found at 1399



Plasmid Map

CrtYB Plasmid Map.png


Sequencing

CrtYB Sequencing.png


This is the sequencing for colony 2. Colony 2 had areas of poor match between both forward and reverse regions. We attribute this to the fact that our gene is far too big for two primers to cover on their own. The middle regions are due to this, and the initial and final regions are due to the trace taking some time to start up.