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Revision as of 01:10, 27 September 2011

Extracellular Domain of E-Cadherin (Mouse)

Cadherin is an adhesive transmembrane and calcium dependent protein that is expressed in many mammalian tissue cells and plays a role in keeping tissues together. It has a domain that anchors the protein to the cytoskeleton of the mammalian cells, an intercellular domain, and five extracellular domain repeats. With the presence of calcium ions, the extracellalur domains become rigid and are able to form homodimers with the same type of cadherin or form heterodimers with different types of cadherin, hence creating cell to cell adhesion.

  • This E-cadherin only contains the extracellular domains of the protein.

We cloned E-cadherin into our plasmid pCTCON2 because pCTCON2 contained the Aga1 and Aga2 protein gene sequences which helped display our E-cadherin and other cloned proteins on the surface of S. cerevisiae.


Cadherin+Ca.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 285
    Illegal BamHI site found at 361
    Illegal BamHI site found at 477
    Illegal BamHI site found at 1401
    Illegal BamHI site found at 1703
    Illegal XhoI site found at 1085
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 405
    Illegal BsaI site found at 947