Difference between revisions of "Part:BBa K642001"
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<partinfo>BBa_K642001 short</partinfo> | <partinfo>BBa_K642001 short</partinfo> | ||
| − | This is the LacI repressor from ''E.coli'' tagged with yeast codon optimized BFP. LacI will bind with high specificity to the the operator ''lacO'' and is inhibited in the presence of IPTG. (1) BFP is a monomeric fluorescent protein | + | This is the LacI repressor from ''E.coli'' tagged with yeast codon optimized BFP. LacI will bind with high specificity to the the operator ''lacO'' and is inhibited in the presence of IPTG. (1) BFP is a monomeric fluorescent protein that has an excitation peak of 399 nm and an emission peak of 465 nm (2). It was yeast codon optimized through DNA synthesis for the purpose of expressing in ''S. cerevisiae'' tagged to various repressors and activators. |
===References=== | ===References=== | ||
Latest revision as of 17:54, 26 September 2011
LacI repressor tagged with yBFP
This is the LacI repressor from E.coli tagged with yeast codon optimized BFP. LacI will bind with high specificity to the the operator lacO and is inhibited in the presence of IPTG. (1) BFP is a monomeric fluorescent protein that has an excitation peak of 399 nm and an emission peak of 465 nm (2). It was yeast codon optimized through DNA synthesis for the purpose of expressing in S. cerevisiae tagged to various repressors and activators.
References
(1) Yansura DG, Henner DJ. (1984). "Use of the Escherichia coli lac repressor and operator to control gene expression in Bacillus subtilis." Proc Natl Acad Sci U S A 81(2): 439-43.
(2) Subach, O. M., I. S. Gundorov, et al. (2008). "Conversion of red fluorescent protein into a bright blue probe." Chem Biol 15(10): 1116-24.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1084
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]