Difference between revisions of "Part:BBa K642006"
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<partinfo>BBa_K642006 short</partinfo> | <partinfo>BBa_K642006 short</partinfo> | ||
| − | This is the cI repressor from phage lambda tagged with yeast codon optimized BFP and a VP16 activation domain. cI will bind to two operators already present in the registry: | + | This is the cI repressor from phage lambda tagged with yeast codon optimized BFP and a VP16 activation domain. cI will bind to two operators already present in the registry: BBa_R0051 & BBa_K105021. BFP is a monomeric fluorescent protein that has an excitation peak of 399 nm and an emission peak of 465 nm (1). It was yeast codon optimized through DNA synthesis for the purpose of expressing in ''S. cerevisiae'' tagged to various repressors and activators. VP16 is a transcription activation domain from the herpes simplex virus that will initiate transcription when fused to a repressor protein. (2) |
===References=== | ===References=== | ||
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(2) Urlinger et al. (2000). "Exploring the sequence space for tetracycline-dependent transcriptional activators: novel mutations yield expanded range and sensitivity." | (2) Urlinger et al. (2000). "Exploring the sequence space for tetracycline-dependent transcriptional activators: novel mutations yield expanded range and sensitivity." | ||
| − | Proc Natl Acad Sci U S A | + | Proc Natl Acad Sci U S A 97(14):7963-8. |
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Latest revision as of 17:42, 26 September 2011
cI repressor tagged with yBFP and a VP16 activation domain
This is the cI repressor from phage lambda tagged with yeast codon optimized BFP and a VP16 activation domain. cI will bind to two operators already present in the registry: BBa_R0051 & BBa_K105021. BFP is a monomeric fluorescent protein that has an excitation peak of 399 nm and an emission peak of 465 nm (1). It was yeast codon optimized through DNA synthesis for the purpose of expressing in S. cerevisiae tagged to various repressors and activators. VP16 is a transcription activation domain from the herpes simplex virus that will initiate transcription when fused to a repressor protein. (2)
References
(1) Subach, O. M., I. S. Gundorov, et al. (2008). "Conversion of red fluorescent protein into a bright blue probe." Chem Biol 15(10): 1116-24.
(2) Urlinger et al. (2000). "Exploring the sequence space for tetracycline-dependent transcriptional activators: novel mutations yield expanded range and sensitivity." Proc Natl Acad Sci U S A 97(14):7963-8.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 711
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]