Difference between revisions of "Part:BBa K575047"

 
 
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This construct is designed to report the presence of PAI2, part of the ''Pseudomonas'' quorum sensing hierarchy. It uses a promoter extracted from a region of the ''Pseudomonas'' genome known to be activated by RhlR/PAI2. When induced with PAI-2 (C4-HSL) at varying concentrations, this construct fluoresced more than the control. Which allows us to use this part as a biosensor for the detection of Pseudomonas Aeruginosa.
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[[Image:S3 Genomic graph.jpg]]__NOTOC__
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<partinfo>BBa_K575047 short</partinfo>
 
<partinfo>BBa_K575047 short</partinfo>
  
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Genomic RhlP. producing GFP along with constiutivley producing RhlR
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This construct contains a LasR/PaI1 (3-oxo-C12-HSL) inducible promoter from the Pseudomonas genome with an RBS and a GFP reporter. There is also constitutive transcription of the LasR receptor protein. Thus, the promoter will activate and fluorescence will be observed in the presence of PAI1.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 01:44, 26 September 2011

This construct is designed to report the presence of PAI2, part of the Pseudomonas quorum sensing hierarchy. It uses a promoter extracted from a region of the Pseudomonas genome known to be activated by RhlR/PAI2. When induced with PAI-2 (C4-HSL) at varying concentrations, this construct fluoresced more than the control. Which allows us to use this part as a biosensor for the detection of Pseudomonas Aeruginosa. S3 Genomic graph.jpg


Genomic RhlR/PAI2 Inducible Promoter + RBS (B0030) + GFP, Cons. Promoter + RBS (B0034) + RhlR

This construct contains a LasR/PaI1 (3-oxo-C12-HSL) inducible promoter from the Pseudomonas genome with an RBS and a GFP reporter. There is also constitutive transcription of the LasR receptor protein. Thus, the promoter will activate and fluorescence will be observed in the presence of PAI1.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1285
    Illegal NheI site found at 1308
    Illegal NotI site found at 304
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1579
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 357
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2054
    Illegal BsaI.rc site found at 1194