Difference between revisions of "Part:BBa K575045:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K575045 short</partinfo>
 
<partinfo>BBa_K575045 short</partinfo>
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===Design Notes===
 
===Design Notes===
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no
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The constitutive promoter is located downstream of the inducible promoter because the reverse alignment may have resulted in unwanted leaky transcription of GFP.
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+
−
 
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===Source===
 
===Source===
  
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from genome and registry
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Other than the genomic promoter, the pieces of this construct are all existing registry parts.
  
 
===References===
 
===References===

Latest revision as of 01:41, 26 September 2011

Genomic LasR/PAI1 Inducible Promoter + RBS (B0034) + GFP, Cons. Promoter + RBS (B0030) + LasR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1281
    Illegal NheI site found at 1304
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1660
    Illegal AgeI site found at 1857
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1190


Design Notes

The constitutive promoter is located downstream of the inducible promoter because the reverse alignment may have resulted in unwanted leaky transcription of GFP.

Source

Other than the genomic promoter, the pieces of this construct are all existing registry parts.

References