Difference between revisions of "Part:BBa K642005"

Revision as of 23:43, 25 September 2011

LacI repressor tagged with yBFP and a VP16 activation domain

This is the LacI repressor from E.coli tagged with yeast codon optimized BFP and a VP16 activation domain. LacI will bind with high specificity to the the operator lacO and is inhibited in the presence of IPTG. (1) BFP is a monomeric fluorescent protein and has an excitation peak of 399 nm and an emission peak of 465 nm (2). It was yeast codon optimized through DNA synthesis for the purpose of expressing in S. cerevisiae tagged to various repressors and activators.

References

(1) Yansura DG, Henner DJ. (1984). "Use of the Escherichia coli lac repressor and operator to control gene expression in Bacillus subtilis." Proc Natl Acad Sci U S A 81(2): 439-43.

(2) Subach, O. M., I. S. Gundorov, et al. (2008). "Conversion of red fluorescent protein into a bright blue probe." Chem Biol 15(10): 1116-24.

(3) Urlinger et al. (2000). "Exploring the sequence space for tetracycline-dependent transcriptional activators: novel mutations yield expanded range and sensitivity." Proc Natl Acad Sci U S A 97(14):7963-8.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1084
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 23:42, 25 September 2011 (view source) Morto077 (Talk \| contribs) (→‎References) ← Older edit		Revision as of 23:43, 25 September 2011 (view source) Morto077 (Talk \| contribs) (→‎References) Newer edit →
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	(2) Subach, O. M., I. S. Gundorov, et al. (2008). "Conversion of red fluorescent protein into a bright blue probe." Chem Biol 15(10): 1116-24.		(2) Subach, O. M., I. S. Gundorov, et al. (2008). "Conversion of red fluorescent protein into a bright blue probe." Chem Biol 15(10): 1116-24.

−	(3) Urlinger et al. (2000). "Exploring the sequence space for tetracycline-dependent transcriptional activators: novel mutations yield expanded range and sensitivity." Proc Natl Acad Sci U S A 5;97(14):7963-8.	+	(3) Urlinger et al. (2000). "Exploring the sequence space for tetracycline-dependent transcriptional activators: novel mutations yield expanded range and sensitivity." Proc Natl Acad Sci U S A 97(14):7963-8.