Difference between revisions of "Part:BBa K642006"

Revision as of 23:36, 25 September 2011

cI repressor tagged with yBFP and a VP16 activation domain

This is the cI repressor from phage lambda tagged with yeast codon optimized BFP and a VP16 activation domain. cI will bind to operators already present in the registry: (BBa_R0051 & BBa_K105021). BFP is a monomeric fluorescent protein that has an excitation peak of 399 nm and an emission peak of 465 nm (1). It was yeast codon optimized through DNA synthesis for the purpose of expressing in S. cerevisiae tagged to various repressors and activators.

References

(1) Subach, O. M., I. S. Gundorov, et al. (2008). "Conversion of red fluorescent protein into a bright blue probe." Chem Biol 15(10): 1116-24.

(2)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 711
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K642006 short</partinfo>
-This is the cI repressor from phage lambda tagged with yeast codon optimized BFP and a VP16 activation domain. cI will bind to operators already present in the registry: (BBa_R0051 & BBa_K105021). BFP is a monomeric fluorescent protein that has an excitation peak of 399 nm and an emission peak of 465 nm (1). It was yeast codon optimized through DNA synthesis for the purpose of expressing in S. cerevisiae tagged to various repressors and activators.
+This is the cI repressor from phage lambda tagged with yeast codon optimized BFP and a VP16 activation domain. cI will bind to operators already present in the registry: (BBa_R0051 & BBa_K105021). BFP is a monomeric fluorescent protein that has an excitation peak of 399 nm and an emission peak of 465 nm (1). It was yeast codon optimized through DNA synthesis for the purpose of expressing in ''S. cerevisiae'' tagged to various repressors and activators.
 ===References===