Difference between revisions of "Part:BBa K575046"
 
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This construct was developed by Northwestern's 2011 iGEM team as part of a ''Pseudomonas Aeruginosa'' detector. The device is designed to fluoresce with GFP in the presence of PAI2 (C4-HSL), one of the ''Pseudomonas'' quorum sensing molecules. The promoter in front of GFP is activated by the combination of PAI2 from the environment and the RhlR receptor (produced by this construct). This promoter was taken from a region of the ''Pseudomonas '' genome known to be activated by RhlR/PAI2. As the graph shows, addition of the autoinducer produces a significant difference in fluorescence compared to the controls. Thus, our construct is successful both in production of RhlR and transcription of GFP in the presence of PAI2.
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[[Image:S4 Genomic graph.jpg]]__NOTOC__
 
[[Image:S4 Genomic graph.jpg]]__NOTOC__
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<partinfo>BBa_K575046 short</partinfo>
 
<partinfo>BBa_K575046 short</partinfo>
  
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Genomic RhlP. producing GFP along with constiutivley producing RhlR
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Continuous expression of RhlR (with RBS B0034), coupled with a RhlR/PAI2 (C4-HSL) inducible promoter, RBS (Part B0034), and a GFP reporter.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 21:34, 25 September 2011

This construct was developed by Northwestern's 2011 iGEM team as part of a Pseudomonas Aeruginosa detector. The device is designed to fluoresce with GFP in the presence of PAI2 (C4-HSL), one of the Pseudomonas quorum sensing molecules. The promoter in front of GFP is activated by the combination of PAI2 from the environment and the RhlR receptor (produced by this construct). This promoter was taken from a region of the Pseudomonas genome known to be activated by RhlR/PAI2. As the graph shows, addition of the autoinducer produces a significant difference in fluorescence compared to the controls. Thus, our construct is successful both in production of RhlR and transcription of GFP in the presence of PAI2.


S4 Genomic graph.jpg


Genomic RhlR/PAI2 Inducible Promoter + RBS (B0034) + GFP, Cons. Promoter + RBS (B0034) + RhlR

Continuous expression of RhlR (with RBS B0034), coupled with a RhlR/PAI2 (C4-HSL) inducible promoter, RBS (Part B0034), and a GFP reporter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1282
    Illegal NheI site found at 1305
    Illegal NotI site found at 304
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1576
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 357
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2051
    Illegal BsaI.rc site found at 1191