Difference between revisions of "Part:BBa K575032:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K575032 short</partinfo> | <partinfo>BBa_K575032 short</partinfo> | ||
<partinfo>BBa_K575032 SequenceAndFeatures</partinfo> | <partinfo>BBa_K575032 SequenceAndFeatures</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | + | The constitutive promoter is located downstream of the inducible promoter because the reverse alignment may have resulted in unwanted leaky transcription of GFP. | |
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===Source=== | ===Source=== | ||
| − | + | The pieces of this construct are all existing registry parts. | |
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===References=== | ===References=== | ||
Latest revision as of 21:29, 25 September 2011
RhlR/PAI2 Inducible Promoter + RBS (B0030) + GFP + Constitutive Promoter + RBS (B0030) + RhlR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 817
Illegal NheI site found at 840 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1114
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1589
Illegal BsaI.rc site found at 726
Design Notes
The constitutive promoter is located downstream of the inducible promoter because the reverse alignment may have resulted in unwanted leaky transcription of GFP.
Source
The pieces of this construct are all existing registry parts.