Difference between revisions of "Part:BBa K642002"
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<partinfo>BBa_K642002 short</partinfo> | <partinfo>BBa_K642002 short</partinfo> | ||
| − | This is the cI repressor from phage lambda tagged with yeast codon optimized BFP. BFP is a monomeric fluorescent protein that has an excitation peak of 399 nm and an emission peak of 465 nm ( | + | This is the cI repressor from phage lambda tagged with yeast codon optimized BFP. cI will bind to operators already present in the registry: (BBa_R0051 & BBa_K105021). BFP is a monomeric fluorescent protein that has an excitation peak of 399 nm and an emission peak of 465 nm (1). It was yeast codon optimized through DNA synthesis for the purpose of expressing in ''S. cerevisiae'' tagged to various repressors and activators. |
===References=== | ===References=== | ||
| − | (1 | + | (1) Subach, O. M., I. S. Gundorov, et al. (2008). "Conversion of red fluorescent protein into a bright blue probe." Chem Biol 15(10): 1116-24. |
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Latest revision as of 20:58, 25 September 2011
cI repressor tagged with yBFP
This is the cI repressor from phage lambda tagged with yeast codon optimized BFP. cI will bind to operators already present in the registry: (BBa_R0051 & BBa_K105021). BFP is a monomeric fluorescent protein that has an excitation peak of 399 nm and an emission peak of 465 nm (1). It was yeast codon optimized through DNA synthesis for the purpose of expressing in S. cerevisiae tagged to various repressors and activators.
References
(1) Subach, O. M., I. S. Gundorov, et al. (2008). "Conversion of red fluorescent protein into a bright blue probe." Chem Biol 15(10): 1116-24.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 711
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]