Difference between revisions of "Part:BBa K642001"

Revision as of 20:48, 25 September 2011

LacI repressor tagged with yBFP

This is the LacI repressor from E.coli tagged with yeast codon optimized BFP. LacI will bind with high specificity to the the operator lacO and is inhibited in the presence of IPTG. (1) BFP is a monomeric fluorescent protein and has an excitation peak of 399 nm and an emission peak of 465 nm (2). It was yeast codon optimized through DNA synthesis for the purpose of expressing in S. cerevisiae tagged to various repressors and activators.

References

(1)Yansura DG, Henner DJ. (1984). "Use of the Escherichia coli lac repressor and operator to control gene expression in Bacillus subtilis." Proc Natl Acad Sci U S A 81(2): 439-43.

(2)Subach, O. M., I. S. Gundorov, et al. (2008). "Conversion of red fluorescent protein into a bright blue probe." Chem Biol 15(10): 1116-24.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Unknown
21
INCOMPATIBLE WITH RFC[21]
Unknown
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 ===References===
-(1)Scrable, H. & Stambrook, P.J. (1997). "Activation of the lac repressor in the transgenic mouse." Genetics 147:  297–304.
+(1)Yansura DG, Henner DJ. (1984). "Use of the Escherichia coli lac repressor and operator to control gene expression in Bacillus subtilis." Proc Natl Acad Sci U S A 81(2): 439-43.
 (2)Subach, O. M., I. S. Gundorov, et al. (2008). "Conversion of red fluorescent protein into a bright blue probe." Chem Biol 15(10): 1116-24.