Difference between revisions of "Part:BBa K642000"
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(1)Hillen, W. & Berens, C. (1994). "Mechanisms underlying expression of Tn10 encoded tetracycline resistance." Annu Rev Microbiol 48:345-69. | (1)Hillen, W. & Berens, C. (1994). "Mechanisms underlying expression of Tn10 encoded tetracycline resistance." Annu Rev Microbiol 48:345-69. | ||
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Revision as of 19:59, 25 September 2011
TetR repressor tagged with yBFP
This is the repressor TetR from the Tn10 tetracycline (Tc) resistance operon of E.coli. It will bind with high specificity to the tetO operator and is inhibited in the presence of doxycycline (1). This repressor is tagged with yeast codon optimized BFP. BFP is a monomeric fluorescent protein and has an excitation peak of 399 nm and an emission peak of 465 nm (2). It was yeast codon optimized through DNA synthesis for the purpose of expressing in S. cerevisiae tagged to various repressors and activators.
References
(1)Hillen, W. & Berens, C. (1994). "Mechanisms underlying expression of Tn10 encoded tetracycline resistance." Annu Rev Microbiol 48:345-69.
(2)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]