Difference between revisions of "Part:BBa K642000"
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<partinfo>BBa_K642000 short</partinfo>
 
<partinfo>BBa_K642000 short</partinfo>
  
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This is the repressor TetR from the Tn''10'' tetracycline (Tc) resistance operon of ''E.coli.'' It will bind with high specificity to the tetO operator and is inhibited in the presence of doxycycline(1). This repressor is tagged with yeast codon optimized BFP. BFP is a monomeric fluorescent protein converted from TagRFP(2). It has an excitation peak of 399 nm and an emission peak of 465 nm(2). It was yeast codon optimized through DNA synthesis for the purpose of expressing in ''S. cerevisiae'' tagged to various repressors and activators.
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This is the repressor TetR from the Tn''10'' tetracycline (Tc) resistance operon of ''E.coli.'' It will bind with high specificity to the tetO operator and is inhibited in the presence of doxycycline (1). This repressor is tagged with yeast codon optimized BFP. BFP is a monomeric fluorescent protein and has an excitation peak of 399 nm and an emission peak of 465 nm (2). It was yeast codon optimized through DNA synthesis for the purpose of expressing in ''S. cerevisiae'' tagged to various repressors and activators.
  
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===References===
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===Usage and Biology===
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(1)Hillen, W. & Berens, C. (1994). "Mechanisms underlying expression of Tn10 encoded tetracycline resistance." Annu Rev Microbiol 48:345-69.
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(2)Subach, O. M., I. S. Gundorov, et al. (2008). "Conversion of red fluorescent protein into a bright blue probe." Chem Biol 15(10): 1116-24.
  
 
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Revision as of 19:58, 25 September 2011

TetR repressor tagged with yBFP

This is the repressor TetR from the Tn10 tetracycline (Tc) resistance operon of E.coli. It will bind with high specificity to the tetO operator and is inhibited in the presence of doxycycline (1). This repressor is tagged with yeast codon optimized BFP. BFP is a monomeric fluorescent protein and has an excitation peak of 399 nm and an emission peak of 465 nm (2). It was yeast codon optimized through DNA synthesis for the purpose of expressing in S. cerevisiae tagged to various repressors and activators.

References

(1)Hillen, W. & Berens, C. (1994). "Mechanisms underlying expression of Tn10 encoded tetracycline resistance." Annu Rev Microbiol 48:345-69. (2)Subach, O. M., I. S. Gundorov, et al. (2008). "Conversion of red fluorescent protein into a bright blue probe." Chem Biol 15(10): 1116-24.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]