Difference between revisions of "Part:BBa K642000"
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<partinfo>BBa_K642000 short</partinfo> | <partinfo>BBa_K642000 short</partinfo> | ||
| − | This is the repressor TetR from the Tn''10'' tetracycline (Tc) resistance operon of ''E.coli.'' | + | This is the repressor TetR from the Tn''10'' tetracycline (Tc) resistance operon of ''E.coli.'' This repressor is tagged with yeast codon optimized BFP. BFP is a monomeric fluorescent protein converted from TagRFP(1). It has an excitation peak of 399 nm and an emission peak of 465 nm(1). It was yeast codon optimized through DNA synthesis for the purpose of expressing in ''S. cerevisiae''. |
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Revision as of 19:42, 25 September 2011
TetR repressor tagged with yBFP
This is the repressor TetR from the Tn10 tetracycline (Tc) resistance operon of E.coli. This repressor is tagged with yeast codon optimized BFP. BFP is a monomeric fluorescent protein converted from TagRFP(1). It has an excitation peak of 399 nm and an emission peak of 465 nm(1). It was yeast codon optimized through DNA synthesis for the purpose of expressing in S. cerevisiae.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]