Difference between revisions of "Part:BBa K249026:Experience"
 
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<I>Experience by Wits-CSIR_SA iGEM2011</I>
 
<I>Experience by Wits-CSIR_SA iGEM2011</I>
 
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<p> Team Lethbridge 2009, used the same theophylline riboswitch (clone 8.1) to generate a riboswitch-CheZ construct. However, this team created a 11 nt insert after the ATG start codon. This result created a TAG stop codon (in the 3rd amino acid position) as well as a frameshift mutation. Therefore it is unlikely that a functional CheZ could have been produced from this system. (see team Lethbridge 2009 below, riboswitch highlighted in yellow, inserted biobrick “scar” region highlighted in violet, ATG start underlined)</p>
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<p> Team Lethbridge 2009, used the Gallivan theophylline riboswitch (clone 8.1) to generate a riboswitch-CheZ construct. However, this team created a 11 nt insert after the ATG start codon. This result created a TAG stop codon (in the 3rd amino acid position) as well as a frameshift mutation. Therefore it is unlikely that a functional CheZ could have been produced from this system. (see team Lethbridge 2009 below, riboswitch highlighted in yellow, inserted biobrick “scar” region highlighted in violet, ATG start underlined)</p>
  
 
<html><img src="https://static.igem.org/mediawiki/2011/6/6c/Wits_Overview_Sequence_Yellow_2.jpg" width="500"></html>
 
<html><img src="https://static.igem.org/mediawiki/2011/6/6c/Wits_Overview_Sequence_Yellow_2.jpg" width="500"></html>

Latest revision as of 17:23, 25 September 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K249026

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BBa_K249026 Experience by Wits-CSIR_SA iGEM2011

Team Lethbridge 2009, used the Gallivan theophylline riboswitch (clone 8.1) to generate a riboswitch-CheZ construct. However, this team created a 11 nt insert after the ATG start codon. This result created a TAG stop codon (in the 3rd amino acid position) as well as a frameshift mutation. Therefore it is unlikely that a functional CheZ could have been produced from this system. (see team Lethbridge 2009 below, riboswitch highlighted in yellow, inserted biobrick “scar” region highlighted in violet, ATG start underlined)

BBa_K249026 - Team Lethbridge iGEM2009

For more detailed analysis of how this part can be improved, visit our results page[http://2011.igem.org/Team:WITS-CSIR_SA/Project/Concept] and the following parts:BBa_K537001 BBa_K537003

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