Difference between revisions of "Part:BBa K249026:Experience"
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| − | <I> | + | <I>Experience by Wits-CSIR_SA iGEM2011</I> |
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| − | + | <p> Team Lethbridge 2009, used the same theophylline riboswitch (clone 8.1) to generate a riboswitch-CheZ construct. However, this team created a 11 nt insert after the ATG start codon. This result created a TAG stop codon (in the 3rd amino acid position) as well as a frameshift mutation. Therefore it is unlikely that a functional CheZ could have been produced from this system. (see team Lethbridge 2009 below, riboswitch highlighted in yellow, inserted biobrick “scar” region highlighted in violet, ATG start underlined)</p> | |
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| + | <html><img src="https://static.igem.org/mediawiki/2011/6/6c/Wits_Overview_Sequence_Yellow_2.jpg" width="500"></html> | ||
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| + | <p>[[BBa_K249026]] - Team Lethbridge iGEM2009/p> | ||
| + | <p>For more detailed analysis of how this part can be improved, visit our results page[http://2011.igem.org/Team:WITS-CSIR_SA/Project/Concept] and the following parts:[[BBa_K537001]] [[BBa_K537003]] </p> | ||
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Revision as of 17:20, 25 September 2011
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K249026
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No review score entered. Experience by Wits-CSIR_SA iGEM2011 |
Team Lethbridge 2009, used the same theophylline riboswitch (clone 8.1) to generate a riboswitch-CheZ construct. However, this team created a 11 nt insert after the ATG start codon. This result created a TAG stop codon (in the 3rd amino acid position) as well as a frameshift mutation. Therefore it is unlikely that a functional CheZ could have been produced from this system. (see team Lethbridge 2009 below, riboswitch highlighted in yellow, inserted biobrick “scar” region highlighted in violet, ATG start underlined)
BBa_K249026 - Team Lethbridge iGEM2009/p> <p>For more detailed analysis of how this part can be improved, visit our results page[http://2011.igem.org/Team:WITS-CSIR_SA/Project/Concept] and the following parts:BBa_K537001 BBa_K537003 |
UNIQ27c6959252f35ca3-partinfo-00000003-QINU