Difference between revisions of "Part:BBa K411001:Experience"

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[http://2010.igem.org/Team:NYMU-Taipei/Experiments/Speedy_switch Experimental Data] using [[Part:BBa_K411003]] to test this part. Works. Downstream translation strength is proportional to the amount of theophylline.
 
[http://2010.igem.org/Team:NYMU-Taipei/Experiments/Speedy_switch Experimental Data] using [[Part:BBa_K411003]] to test this part. Works. Downstream translation strength is proportional to the amount of theophylline.
 
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<partinfo>BBa_K411001 AddReview number</partinfo>
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<I>Wits-CSIR_SA iGEM2011</I>
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<p>Team NYMU Taipei assumed that a created theophylline riboswitch (Gallivan lab clone 8.1) biobrick could be fused to a GFP coding region by standard biobrick assembly techniques. While it is possible to do this, we feel that the resulting 6 nt scar sequence insertion increases the distance between the RBS and the ATG start codon, a result which is likely to cause a decrease in riboswitch efficiency  (see team Taipei switch below, riboswitch highlighted in yellow, inserted biobrick “scar” region highlighted in violet, ATG start underlined). In fact, Taipei 2010 did show data which showed only a 2.5 fold increase in gene activation at approx. 4 mM theophylline. Compared to the 36-fold activation at 1mM theophylline by Gallivan lab, this seems too low! We therefore feel that this riboswitch is not behaving efficiently.</p>
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<img src="https://static.igem.org/mediawiki/2011/6/63/Wits_Overview_Sequence_Yellow.jpg" width="500">
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<p><b>Figure 8.</b> <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K411001">BBa_K411001</a>,<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K411003">BBa_K411003</a> - Team NYMU-Taipei iGEM2010</p>
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<!-- DON'T DELETE --><partinfo>BBa_K411001 EndReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K411001 EndReviews</partinfo>

Revision as of 16:34, 25 September 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K411001

User Reviews

UNIQcb920dc4a9b4a64c-partinfo-00000000-QINU

No review score entered. NYMU-Taipei iGEM10

[http://2010.igem.org/Team:NYMU-Taipei/Experiments/Speedy_switch Experimental Data] using Part:BBa_K411003 to test this part. Works. Downstream translation strength is proportional to the amount of theophylline.

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No review score entered. Wits-CSIR_SA iGEM2011 |width='60%' valign='top'|

Team NYMU Taipei assumed that a created theophylline riboswitch (Gallivan lab clone 8.1) biobrick could be fused to a GFP coding region by standard biobrick assembly techniques. While it is possible to do this, we feel that the resulting 6 nt scar sequence insertion increases the distance between the RBS and the ATG start codon, a result which is likely to cause a decrease in riboswitch efficiency (see team Taipei switch below, riboswitch highlighted in yellow, inserted biobrick “scar” region highlighted in violet, ATG start underlined). In fact, Taipei 2010 did show data which showed only a 2.5 fold increase in gene activation at approx. 4 mM theophylline. Compared to the 36-fold activation at 1mM theophylline by Gallivan lab, this seems too low! We therefore feel that this riboswitch is not behaving efficiently.


<img src="Wits_Overview_Sequence_Yellow.jpg" width="500">

Figure 8. <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K411001">BBa_K411001</a>,<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K411003">BBa_K411003</a> - Team NYMU-Taipei iGEM2010


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UNIQcb920dc4a9b4a64c-partinfo-00000003-QINU