Difference between revisions of "Part:BBa K611012"
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<partinfo>BBa_K611012 short</partinfo> | <partinfo>BBa_K611012 short</partinfo> | ||
| − | This part is used to characterize mutants of BBa_R0040 (pTet). | + | This part is used to characterize mutants of BBa_R0040 (pTet). A mutant of BBa_R0040 can be ligated before this part (on the 5' end) by cutting this plasmid at EcoRI and XbaI and the mutant promoter at EcoRI and SpeI. Several of the mutant's properties can then be measured through the expression of GFP. Certain characteristics of the mutant, such as the binding of the TetR repressor, can be measured after inducing the pBad promoter with arabinose. Concentration of the repressor can be varied through different arabinose concentrations as long as AraC is present. |
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Latest revision as of 01:25, 25 September 2011
pTet Characterization Construct
This part is used to characterize mutants of BBa_R0040 (pTet). A mutant of BBa_R0040 can be ligated before this part (on the 5' end) by cutting this plasmid at EcoRI and XbaI and the mutant promoter at EcoRI and SpeI. Several of the mutant's properties can then be measured through the expression of GFP. Certain characteristics of the mutant, such as the binding of the TetR repressor, can be measured after inducing the pBad promoter with arabinose. Concentration of the repressor can be varied through different arabinose concentrations as long as AraC is present.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1008
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 948
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 662