Difference between revisions of "Part:BBa K530037"

 
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TRP1
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Yeast shuttle vector for propagation in ''E. coli'' and transformation into ''S. cerevisiae''. Contains an Ampicillin resistance marker for ''E. coli'' and TRP1 selectable marker for ''S. cerevisiae''.  Contains EcoRI, NheI, SpeI, and PstI in the multiple cloning site, in that order. For assembly, this vector differs from a Standard Assembly vector by replacing XbaI with NheI. This vector is a 2micron episomal yeast vector.
  
 
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<partinfo>BBa_K530037 parameters</partinfo>
 
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===Plasmid Map===
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[[Image:pRS424.png]]

Revision as of 01:09, 25 September 2011

pRSBB424 TRP1 2Micron Episomal Yeast Shuttle Vector

Yeast shuttle vector for propagation in E. coli and transformation into S. cerevisiae. Contains an Ampicillin resistance marker for E. coli and TRP1 selectable marker for S. cerevisiae. Contains EcoRI, NheI, SpeI, and PstI in the multiple cloning site, in that order. For assembly, this vector differs from a Standard Assembly vector by replacing XbaI with NheI. This vector is a 2micron episomal yeast vector.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 551
    Illegal XbaI site found at 5076
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 551
    Illegal XbaI site found at 5076
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 551
    Illegal XbaI site found at 5076
    Illegal NgoMIV site found at 1566
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3246
    Illegal SapI site found at 2163


Plasmid Map

PRS424.png