Difference between revisions of "Part:BBa K590062"

Latest revision as of 21:36, 24 September 2011

Gli1-ADC HivC-AAR Construct

This part encodes Gli1-Aldehyde Decarbonylase and HivC-Acyl-ACP Reductase Zn-finger fusion proteins under the control of a lac-induced promoter. These co-localize when co-expressed with Slovenia's 2010 DNA Program, and produce alkanes.

Usage and Biology

Our attempt at zinc finger localization resulted in an alkane production of approximately 7 mg/L, which is significantly less than our delocalized PetroBrick standard, which produced around 36 mg alkanes per liter. For more information, refer to [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team wiki].

We chose to integrate the use of DNA localization via zinc finger proteins to colocalize ADC and AAR to increase system flux.

Our gas chromatography-mass spectroscopy (GC-MS) data shows that the alkane yields from zinc finger were low. For more info, refer to 2011 UW iGEM Team wiki

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Unknown
21
INCOMPATIBLE WITH RFC[21]
Unknown
23
INCOMPATIBLE WITH RFC[23]
Unknown
25
INCOMPATIBLE WITH RFC[25]
Unknown
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K590062 short</partinfo>
-This part containing Gli1-ADC and HivC-AAR fusion proteins unde a lac-induced promoter for alkane production when co-expressed with Slovenia's 2010 [https://parts.igem.org/wiki/index.php?title=Part:BBa_K323066 DNA Program].
+This part encodes Gli1-[https://parts.igem.org/wiki/index.php?title=Part:BBa_K590031 Aldehyde Decarbonylase] and HivC-[https://parts.igem.org/wiki/index.php?title=Part:BBa_K590032 Acyl-ACP Reductase] Zn-finger fusion proteins under the control of a lac-induced promoter. These co-localize when co-expressed with Slovenia's 2010 [https://parts.igem.org/wiki/index.php?title=Part:BBa_K323066 DNA Program], and produce alkanes.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+Our attempt at zinc finger localization resulted in an alkane production of approximately 7 mg/L, which is significantly less than our delocalized PetroBrick standard, which produced around 36 mg alkanes per liter.
+For more information, refer to [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team wiki].
+[[Image:Washington_ZnF_Localization_Image2.png‎|left|430px|thumb|We chose to integrate the use of DNA localization via zinc finger proteins to colocalize ADC and AAR to increase system flux.]]
+[[Image:Washington2011_Alkane_Production_Localization_Graph1.png‎ |right|440px|thumb|Our gas chromatography-mass spectroscopy (GC-MS) data shows that the alkane yields from zinc finger were low. For more info, refer to 2011 UW iGEM Team wiki]]
 <!-- -->