Difference between revisions of "Part:BBa K590051"

Revision as of 21:13, 24 September 2011

Acyl-ACP Reductase to Aldehyde Decarbonylase (AAR-ADC) Fusion

This part encodes a direct gene fusion product of the two enzymes, Acyl-ACP Reductase(AAR) and Aldehyde Decarbonylase (ADC), in a linear configuration with a glycine-serine linker for co-localization optimization of ADC alkane production developed by the [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team].

Usage and Biology

Direct fusion of two proteins using a glycine-serine linker.

Contrary to what was expected, our GCMS data demonstrated that direct fusion has a negative effect on alkane production.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K590051 short</partinfo>
-This part encodes a direct gene fusion product of the two enzymes, [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590032 Acyl-ACP Reductase(AAR)] and  [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590031 Aldehyde Decarbonylase (ADC)], in a linear configuration with a linker for co-localization optimization of ADC alkane production developed by the [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team].
+This part encodes a direct gene fusion product of the two enzymes, [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590032 Acyl-ACP Reductase(AAR)] and  [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590031 Aldehyde Decarbonylase (ADC)], in a linear configuration with a glycine-serine linker for co-localization optimization of ADC alkane production developed by the [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team].
 ===Usage and Biology===
-[[Image:Washington2011_Direct_Fusion_Localization_Image.png|left|400px|thumb|]]
+[[Image:Washington2011_Direct_Fusion_Localization_Image.png|left|430px|thumb|Direct fusion of two proteins using a glycine-serine linker.]]
-[[Image:Washington2011_Alkane_Production_Localization_Graph1.png‎ |right|400px|thumb|]]
+[[Image:Washington2011_Alkane_Production_Localization_Graph1.png‎ |right|430px|thumb|Contrary to what was expected, our GCMS data demonstrated that direct fusion has a negative effect on alkane production.]]
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