Difference between revisions of "Part:BBa K542009"
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<partinfo>BBa_K542009 short</partinfo> | <partinfo>BBa_K542009 short</partinfo> | ||
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Made by assembling [https://parts.igem.org/Part:BBa_J04500 BBa_J04500] with [https://parts.igem.org/Part:BBa_K346007 BBa_K346007]. | Made by assembling [https://parts.igem.org/Part:BBa_J04500 BBa_J04500] with [https://parts.igem.org/Part:BBa_K346007 BBa_K346007]. | ||
− | Although characterized by the Peking 2010 team, the part submitted was a basic part with no promoter or RBS (https://parts.igem.org/Part:BBa_K346007 BBa_K346007). The pLacI promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010 BBa_R0010]) and RBS (https://parts.igem.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034) was added. | + | Although characterized by the Peking 2010 team, the part submitted was a basic part with no promoter or RBS ([https://parts.igem.org/Part:BBa_K346007 BBa_K346007]). The pLacI promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010 BBa_R0010]) and RBS (https://parts.igem.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034) was added. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 16:11, 24 September 2011
pLacI Regulated AG43
Made by assembling BBa_J04500 with BBa_K346007.
Although characterized by the Peking 2010 team, the part submitted was a basic part with no promoter or RBS (BBa_K346007). The pLacI promoter (BBa_R0010) and RBS (https://parts.igem.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034) was added.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 935
Illegal NgoMIV site found at 2159
Illegal NgoMIV site found at 2669
Illegal NgoMIV site found at 2690
Illegal AgeI site found at 1685
Illegal AgeI site found at 2429
Illegal AgeI site found at 2843
Illegal AgeI site found at 3085 - 1000COMPATIBLE WITH RFC[1000]