Difference between revisions of "Part:BBa K676003:Design"

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Forward: TAGTTCGAATTCTCTAGACAGTTCATTTTTCTCAACG
 
Forward: TAGTTCGAATTCTCTAGACAGTTCATTTTTCTCAACG
 +
 
Reverse: ATCATCCTGCAGACTAGTACGGGGTAATGCTTTTTAC
 
Reverse: ATCATCCTGCAGACTAGTACGGGGTAATGCTTTTTAC
  

Revision as of 23:47, 23 September 2011

TyrT Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers designed for PCR:

Forward: TAGTTCGAATTCTCTAGACAGTTCATTTTTCTCAACG

Reverse: ATCATCCTGCAGACTAGTACGGGGTAATGCTTTTTAC

Final PCR product is 115 bp

Source

Escherichia Coli K-12 genome - from 1286864 to 1286948 bp

References

[EcoCyc11] Keseler, I.M., Collado-Vides, J., Santos-Zavaleta, A., Peralta-Gil, M., Gama-Castro, S., Muniz-Rascado, L., Bonavides-Martinez, C., Paley, S., Krummenacker, M., Altman, T., Kaipa, P., Spaulding, A., Pacheco, J., Latendresse, M., Fulcher, C., Sarker, M., Shearer, A.G., Mackie, A., Paulsen, I., Gunsalus, R.P., and Karp, P.D., EcoCyc: a comprehensive database of Escherichia coli biology Nucleic Acids Research 39:D583-590 2011.