Difference between revisions of "Part:BBa K606033"
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===Usage and Biology===
 
===Usage and Biology===
  
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The T7 RNA polymerases have two stops codons at the begening of its sequence. The protein cannot be expressed unless a tRNA amber supressor is expressed in the cell. (for instance K606034 for B. subtilis). This strategy can be use to create an and gate. If you want then the signal to be amplify, you can unse both K606018 or K606035 for reporting.
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The T7 RNA polymerase have two stops codons at the begining of its sequence. The protein cannot be expressed unless a tRNA amber supressor is expressed in the cell. (for instance K606034 for B. subtilis). This strategy can be use to create an AND gate. If the signal is to be amplified, K606018 or K606035 can be used for reporting.
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 18:54, 23 September 2011

pVeg SpoVG T7amber double terminator.

T7 RNA polymerase tyrosine amber mutated expression casette.


Usage and Biology

The T7 RNA polymerase have two stops codons at the begining of its sequence. The protein cannot be expressed unless a tRNA amber supressor is expressed in the cell. (for instance K606034 for B. subtilis). This strategy can be use to create an AND gate. If the signal is to be amplified, K606018 or K606035 can be used for reporting.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]