Difference between revisions of "Part:BBa K606029"

Latest revision as of 18:15, 23 September 2011

RBS SpoVG GFPmut3b T7terminator

This part is a GFP reporter system for B. Subtilis but can also work in E. coli. The terminator cloned downstream is the one for the T7 RNA polymerase but can also be used for endogenic RNA polymerase.

Usage and Biology

This part has been design to be under the control of a T7 promoter and polymerased by the T7 RNA polymerase for which it has been characterised. It can also be used sucessfully in E. coli with a standard promoter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 886
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 662

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K606029 short</partinfo>
-This is a GFP expression system for B. Subtilis that is dont to be polymerazed by the T7 polymerase. This have to be activated by the pT7 promoter I712074. The RBS used is SpoVG, that is design for a strong expression in B. Subtilis.
+This part is a GFP reporter system for B. Subtilis but can also work in E. coli. The terminator cloned downstream is the one for the T7 RNA polymerase but can also be used for  endogenic RNA polymerase.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
-<!-- -->
+This part has been design to be under the control of a T7 promoter and polymerased by the T7 RNA polymerase for which it has been characterised. It can also be used  sucessfully in E. coli with a standard promoter.
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K606029 SequenceAndFeatures</partinfo>