Difference between revisions of "Part:BBa K606029"

(→‎Usage and Biology)
(→‎Usage and Biology)
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===Usage and Biology===
 
===Usage and Biology===
  
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This part has been design to be under the control of a T7 promoter and polymerased by the T7 RNA polymerase for which it has been characterizer. It can also used it sucessfully in E. coli with a standard promoter.
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This part has been design to be under the control of a T7 promoter and polymerased by the T7 RNA polymerase for which it has been characterised. It can also be used sucessfully in E. coli with a standard promoter.
  
  

Revision as of 18:14, 23 September 2011

RBS SpoVG GFPmut3b T7terminator

This part is a GFP reporter system for B. Subtilis but can also work in E. coli. The terminator cloned downstream is the one for the T7 RNA polymerase, but can be used also for the endogenic RNA polymerase.


Usage and Biology

This part has been design to be under the control of a T7 promoter and polymerased by the T7 RNA polymerase for which it has been characterised. It can also be used sucessfully in E. coli with a standard promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 886
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 662