Difference between revisions of "Part:BBa K606025"

 
 
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<partinfo>BBa_K606025 short</partinfo>
 
<partinfo>BBa_K606025 short</partinfo>
  
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YFP:tetR protein fusion.  
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YFP:tetR is a recombinant fusion protein. It is composed of Yellow Fluorescent Protein (YFP) and Tetracycline Repressor Protein (tetR) that can bind to the tet operator sequence (tetO). Using the tetO array  (<partinfo>BBa_K606026</partinfo>) composed of a 3,7kb tetO sequence, we can concentrate YFP:tetR in a few loci and increase the fluorescence sensibility.<br> <br>Origins of YFP:tetR and tetO Array come from François-Xavier Barre, Andrew Wright and Dave Lane (Kinetics of plasmid segregation, Molecular Microbiology, 2004)
  
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Use to focalise fluorescence in few spots.
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===Usage and Biology===
  
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It binds on tetO array (<partinfo>BBa_K606026</partinfo>) (containing 10kb of tetO).
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The combination of both parts K606025 and K606026 can be use to concentrate a fluorescence signal and make a smaller nmber of fluorescent proteins visible under the microscope. See the experiments page for characterization and picture of what can be seen under the microscope.
  
  
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===Usage and Biology===
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K606025 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K606025 SequenceAndFeatures</partinfo>

Latest revision as of 17:59, 23 September 2011

YFP:tetR recombinant fusion protein

YFP:tetR is a recombinant fusion protein. It is composed of Yellow Fluorescent Protein (YFP) and Tetracycline Repressor Protein (tetR) that can bind to the tet operator sequence (tetO). Using the tetO array (BBa_K606026) composed of a 3,7kb tetO sequence, we can concentrate YFP:tetR in a few loci and increase the fluorescence sensibility.

Origins of YFP:tetR and tetO Array come from François-Xavier Barre, Andrew Wright and Dave Lane (Kinetics of plasmid segregation, Molecular Microbiology, 2004)

Usage and Biology

The combination of both parts K606025 and K606026 can be use to concentrate a fluorescence signal and make a smaller nmber of fluorescent proteins visible under the microscope. See the experiments page for characterization and picture of what can be seen under the microscope.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 631
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]