Difference between revisions of "Part:BBa K590061"
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<partinfo>BBa_K590061 short</partinfo> | <partinfo>BBa_K590061 short</partinfo> | ||
− | [http://2011.igem.org/Team:Washington University of Washington 2011 iGEM Team] has made a synthesized version of the Lux E gene from the [http://2010.igem.org/Team:Cambridge/Bioluminescence/Bacterial_Luciferases Cambridge 2010 LuxBrick]. It was | + | [http://2011.igem.org/Team:Washington University of Washington 2011 iGEM Team] has made a synthesized version of the Lux E gene from the [http://2010.igem.org/Team:Cambridge/Bioluminescence/Bacterial_Luciferases Cambridge 2010 LuxBrick]. It was optimized to have cloning-friendly GC content and systemized to fit in ''Escherichia coli''. |
===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 01:39, 23 September 2011
LuxE; standalone, E. Coli codon optimized
[http://2011.igem.org/Team:Washington University of Washington 2011 iGEM Team] has made a synthesized version of the Lux E gene from the [http://2010.igem.org/Team:Cambridge/Bioluminescence/Bacterial_Luciferases Cambridge 2010 LuxBrick]. It was optimized to have cloning-friendly GC content and systemized to fit in Escherichia coli.
Usage and Biology
[http://2011.igem.org/Team:Washington University of Washington 2011 iGEM Team] has tried to use this part along with Lux C and Lux D to engineer alternative routes for fatty aldehyde production, mainly tetradecanal.Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 115