Difference between revisions of "Part:BBa K590034"
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<partinfo>BBa_K590034 short</partinfo>
 
<partinfo>BBa_K590034 short</partinfo>
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[http://2011.igem.org/Team:Washington University of Washington 2011 iGEM Team] has some evidence to indicate cause ''Escherichia coli'' to produce linear, even-length chain fatty acids.
 
  
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 01:13, 23 September 2011

FabH2

Usage and Biology

FabH2 is from Bacillus subtilis. The FabH family of proteins initiates fatty acid elongation by converting an Acyl-CoA into an Acyl-ACP, with is extended by 2 carbon units to form longer chain length fatty acids. Normally, FabH proteins use a simple 2-carbon acetyl-CoA to start fatty acid biosynthesis, resulting in linear fatty acids. However, FabH2 can also use Isobutyryl-CoA, Isovaleryl-CoA, and 2-Methylbutyryl-CoA (products from Valine, Leucine, and Isoleucine degredation, resulting in 2-methyl branched fatty acid production.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 310
    Illegal AgeI site found at 949
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 355