Difference between revisions of "Part:BBa K590053"
Line 9: | Line 9: | ||
[[Image:Washinton_AARADCGC-MS.png|left|400px|thumb|GC plot showing that alkanes are only produced when both ADC and AAR are expressed.]] | [[Image:Washinton_AARADCGC-MS.png|left|400px|thumb|GC plot showing that alkanes are only produced when both ADC and AAR are expressed.]] | ||
− | [[Image:Washington_Redesign_Data2.png|right| | + | [[Image:Washington_Redesign_Data2.png|right|430px|thumb|Mutant ADCs are co-expressed with AARs under optimized conditions.]] |
+ | |||
+ | |||
Revision as of 19:19, 22 September 2011
RrD_PSB1C3-High constitutive
This part consists of Acyl-ACP Reductase, ribosomal binding site, Aldehyde Decarbonylase under the control of high constitutive promoter for alkane production.
Usage and Biology
When wild type Aldehyde Decarbonylase is co-expressed with Acyl-ACP Reductase, odd chain length alkanes is produced from the cell's fatty acid biosynthetic pathway. Alkane production is enhanced when growing expression strains using the growth conditions developed by the [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 470
Illegal NheI site found at 493 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1584
Illegal XhoI site found at 2366 - 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000COMPATIBLE WITH RFC[1000]