Difference between revisions of "Part:BBa K590041"
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<partinfo>BBa_K590041 short</partinfo> | <partinfo>BBa_K590041 short</partinfo> | ||
| − | + | [http://2011.igem.org/Team:Washington University of Washington 2011 iGEM Team] has designed this mutant Aldehyde Decarbonylase (ADC) to modify the specificity of the wild type ADC so that when it works together with Acyl-ACP Reductase (AAR), it will produce more C13 and C15 alkanes. For more information, refer to the fully assembled brick, [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590058 BBa_K590058]. | |
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | This mutant Aldehyde Decarbonylase has amino acid changes from tyrosine , Y, to tryptophan, W, at residue 21 and from cysteine, C, to isoleucine, I, at residue 70. | + | This mutant Aldehyde Decarbonylase has amino acid changes from tyrosine, Y, to tryptophan, W, at residue 21 and from cysteine, C, to isoleucine, I, at residue 70. |
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Latest revision as of 18:57, 22 September 2011
Aldehyde Decarbonylase_Y21W, C70I
[http://2011.igem.org/Team:Washington University of Washington 2011 iGEM Team] has designed this mutant Aldehyde Decarbonylase (ADC) to modify the specificity of the wild type ADC so that when it works together with Acyl-ACP Reductase (AAR), it will produce more C13 and C15 alkanes. For more information, refer to the fully assembled brick, BBa_K590058.
Usage and Biology
This mutant Aldehyde Decarbonylase has amino acid changes from tyrosine, Y, to tryptophan, W, at residue 21 and from cysteine, C, to isoleucine, I, at residue 70.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 730
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]