Difference between revisions of "Part:BBa K606029"
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<partinfo>BBa_K606029 short</partinfo>
 
<partinfo>BBa_K606029 short</partinfo>
  
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This is a GFP expression system for B. Subtilis that is dont to be polymerazed by the T7 polymerase. This have to be activated by the pT7 promoter I712074. The RBS used is SpoVG, that is design for a strong expression in B. Subtilis.
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This part is a GFP reporter system for B. Subtilis, but that can work in E. coli. The promoter cloned downstream is the one of the T7 RNA polymerase. This stop can also work work standard RNA polymerases.
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
  
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This part has been design to be under the control of a T7 promoter and polymerased by the T7 RNA polymerase for which it has been characterizer. However, we also used it sucessfully in E. coli with a standard promoter.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K606029 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K606029 SequenceAndFeatures</partinfo>

Revision as of 16:18, 22 September 2011

RBS SpoVG GFPmut3b T7terminator

This part is a GFP reporter system for B. Subtilis, but that can work in E. coli. The promoter cloned downstream is the one of the T7 RNA polymerase. This stop can also work work standard RNA polymerases.


Usage and Biology

This part has been design to be under the control of a T7 promoter and polymerased by the T7 RNA polymerase for which it has been characterizer. However, we also used it sucessfully in E. coli with a standard promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 886
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 662