Difference between revisions of "Part:BBa J100031:Experience"
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| − | Experiment testing T7 C promoter against 2 controls and one other promoter. All 6 tubes contained E.coli with the promoters in plasmids, also containing LB. Tube A contained T7 C Promoter, Tube B contained T7 C plus the inducer IPTG. The IPTG inducer binds to the repressor and inactivates it. This inducer is frequently used with E.coli and pLac promoter. Tube C contained J100028, an insert, but this tube did not have a promoter, and was a negative control. Tube D was a positive control, containing pTet, a promoter that known to work with RFP protein and E.coli. Tubes E and F contained the promoter pLac I, which is known to work with E.coli. Tube E also contained the inducer IPTG. The graph represents fluorescence of the Red Fluorescent Protein gene, as a function of cell density. These results were found using a spectrophotometer. | + | Experiment testing T7 C promoter against 2 controls and one other promoter. All 6 tubes contained E.coli with the promoters in plasmids, also containing LB. Tube A contained T7 C Promoter, Tube B contained T7 C plus the inducer IPTG. The IPTG inducer binds to the repressor and inactivates it. This inducer is frequently used with E.coli and pLac promoter. Tube C contained J100028, an insert, but this tube did not have a promoter, and was a negative control. Tube D was a positive control, containing pTet, a promoter that known to work with RFP protein and E.coli. Tubes E and F contained the promoter pLac I, which is known to work with E.coli. Tube E also contained the inducer IPTG. The graph represents fluorescence of the Red Fluorescent Protein gene, as a function of cell density. These results were found using a spectrophotometer. Tube A fluoresced more than the negative control, but in Tube B the inducer did not have any benefit. Further experiments could be done to make sure that human or measurement errors were not a factor in the T7 C promoter not functioning. |
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 14:01, 22 September 2011
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Applications of BBa_J100031
Experiment testing T7 C promoter against 2 controls and one other promoter. All 6 tubes contained E.coli with the promoters in plasmids, also containing LB. Tube A contained T7 C Promoter, Tube B contained T7 C plus the inducer IPTG. The IPTG inducer binds to the repressor and inactivates it. This inducer is frequently used with E.coli and pLac promoter. Tube C contained J100028, an insert, but this tube did not have a promoter, and was a negative control. Tube D was a positive control, containing pTet, a promoter that known to work with RFP protein and E.coli. Tubes E and F contained the promoter pLac I, which is known to work with E.coli. Tube E also contained the inducer IPTG. The graph represents fluorescence of the Red Fluorescent Protein gene, as a function of cell density. These results were found using a spectrophotometer. Tube A fluoresced more than the negative control, but in Tube B the inducer did not have any benefit. Further experiments could be done to make sure that human or measurement errors were not a factor in the T7 C promoter not functioning.
User Reviews
UNIQ50f0862256c8041e-partinfo-00000000-QINU UNIQ50f0862256c8041e-partinfo-00000001-QINU