Difference between revisions of "Part:BBa J100031:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_J100031=== | ===Applications of BBa_J100031=== | ||
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| + | [[Image:T7CPromoter.png]] | ||
| + | Experiment testing T7 C promoter against 2 controls and one other promoter. All 6 tubes contained E.coli with the promoters in plasmids, also containing LB. Tube A contained T7 C Promoter, Tube B contained T7 C plus the inducer IPTG. The IPTG inducer binds to the repressor and inactivates it. This inducer is frequently used with E.coli and pLac promoter. Tube C contained J100028, an insert, but this tube did not have a promoter, and was a negative control. Tube D was a positive control, containing pTet, a promoter that known to work with RFP protein and E.coli. Tubes E and F contained the promoter pLac I, which is known to work with E.coli. Tube E also contained the inducer IPTG. The graph represents fluorescence of the Red Fluorescent Protein gene, as a function of cell density. These results were found using a spectrophotometer. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 13:50, 22 September 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_J100031
Experiment testing T7 C promoter against 2 controls and one other promoter. All 6 tubes contained E.coli with the promoters in plasmids, also containing LB. Tube A contained T7 C Promoter, Tube B contained T7 C plus the inducer IPTG. The IPTG inducer binds to the repressor and inactivates it. This inducer is frequently used with E.coli and pLac promoter. Tube C contained J100028, an insert, but this tube did not have a promoter, and was a negative control. Tube D was a positive control, containing pTet, a promoter that known to work with RFP protein and E.coli. Tubes E and F contained the promoter pLac I, which is known to work with E.coli. Tube E also contained the inducer IPTG. The graph represents fluorescence of the Red Fluorescent Protein gene, as a function of cell density. These results were found using a spectrophotometer.
User Reviews
UNIQ56edd1e514dd0d72-partinfo-00000000-QINU UNIQ56edd1e514dd0d72-partinfo-00000001-QINU