Difference between revisions of "Part:BBa K590001"

Latest revision as of 05:35, 22 September 2011

mamE, from Magnetospirillum magneticum

This part was extracted from the genome of Magnetospirillum magneticum strain AMB-1 by [http://2011.igem.org/Team:Washington UW iGEM Team 2011]

Usage and Biology

mamE is 2172bp in length and its gene number is AMB-0963. It was reported as membrane-bound serine protease required for magnetite formation; it might also associate with the localization of other magnetosome proteins.

The image below is the gel of mamE with expected length of 2172bp.

1kb ladder (left), mamE (right)

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 884
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 884
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 2084
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 884
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 884
Illegal AgeI site found at 1558
Illegal AgeI site found at 1973
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K590001 short</partinfo>
-AMB#0963; Gene function: membrane-bound serine protease required for magnetite formation; might control the localization of other magnetosome proteins
+This part was extracted from the genome of ''Magnetospirillum magneticum'' strain AMB-1 by [http://2011.igem.org/Team:Washington UW iGEM Team 2011]
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+mamE is 2172bp in length and its gene number is AMB-0963. It was reported as membrane-bound serine protease required for magnetite formation; it might also associate with the localization of other magnetosome proteins.
+The image below is the gel of mamE with expected length of 2172bp.
+[[Image:Igem2011 E gel.png|thumb|center|1kb ladder (left), mamE (right)]]
 <!-- -->