Difference between revisions of "Part:BBa K676001"
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The strong gyrase site clones from Mu bacteriophage. | The strong gyrase site clones from Mu bacteriophage. | ||
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The Mu phage Gyrase Binding sites serve as a high affinity binding site for the DNA gyrase enzymes. The site will be cleaved preferentially by the GyrA and resulting in an introduction of linking number difference into the DNA by the topoisomerase. | The Mu phage Gyrase Binding sites serve as a high affinity binding site for the DNA gyrase enzymes. The site will be cleaved preferentially by the GyrA and resulting in an introduction of linking number difference into the DNA by the topoisomerase. | ||
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By introducing the Mu GBS into the target plasmids, we achieved a higher level of supercoiling as well as obtaining a more uniformly supercoiled products . Highly supercoiled plasmids are able to resist shear stress better and this property plays an important role in large scale plasmid DNA manufacturing as well as gene therapy delivery. | By introducing the Mu GBS into the target plasmids, we achieved a higher level of supercoiling as well as obtaining a more uniformly supercoiled products . Highly supercoiled plasmids are able to resist shear stress better and this property plays an important role in large scale plasmid DNA manufacturing as well as gene therapy delivery. | ||
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| + | ===Usage and Biology=== | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 04:21, 22 September 2011
Gyrase Binding Site from Mu Bacteriophage
The strong gyrase site clones from Mu bacteriophage.
The Mu phage Gyrase Binding sites serve as a high affinity binding site for the DNA gyrase enzymes. The site will be cleaved preferentially by the GyrA and resulting in an introduction of linking number difference into the DNA by the topoisomerase.
Apparently some bacteriophages exploited the GBS to allow them to replicate successfully in their host. For example, Mu – bacteriophage has a strong gyrase site or GBS in the central region of the 37.2kbp Mu phage genome. From Pato(1990) it was found out that the wild type phage can carry out replication effectively compare to the mutant which lacks the GBS after the heat induction of the lysogens .
By introducing the Mu GBS into the target plasmids, we achieved a higher level of supercoiling as well as obtaining a more uniformly supercoiled products . Highly supercoiled plasmids are able to resist shear stress better and this property plays an important role in large scale plasmid DNA manufacturing as well as gene therapy delivery.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 191
- 1000COMPATIBLE WITH RFC[1000]