Difference between revisions of "Part:BBa K606036:Experience"
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{| border="1" class="wikitable" style="text-align: center;"
 
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|+T7 autoloop in E.coli at 37°C
 
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|[[File:T7 autoloop ctrl-25.jpg|450px|thumb|center|E.coli T7 autoloop without terminator at 37°C  (trans image)]]
 
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|[[File:T7 autoloop ctrl-26.jpg|450px|thumb|center|E.coli T7 autoloop without terminator at 37°C  (gfp image)]]
 
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|-
 
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|[[File:T7_loop_1.jpg|450px|thumb|center|E.coli T7 autoloop with terminator  at 37°C  (trans image)]]
 
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|[[File:T7 loop 1 gfp.jpg|450px|thumb|center|E.coli T7 autoloop with terminator at 37°C  (gfp image)]]
 
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|-
 
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|[[File:T7 autoloop ctrl-10.jpg|450px|thumb|center|E.coli negative control  at 37°C  (trans image)]]
 
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|[[File:T7 autoloop ctrl-11.jpg|450px|thumb|center|E.coli negative control at 37°C  (gfp image)]]
 
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|}
 
  
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These pictures show that the T7 GFP autoloop system is efficient since some cells are glowing with GFP fluorescence. Thus, we can conclude that the T7 polymerase is activated.
 
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Finally, we notice there is no difference between the GFP autoloop with and without terminator before the T7 promoter, we can interpret this due to this terminator is a B.subtilis terminator, moreover, we know that this E.coli strain has 4 terminators.
 
  
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</html>
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[[File:T7 autoloop ctrl-25.jpg|450px|thumb|center|E.coli T7 autoloop without terminator at 37°C  (trans image) ]]
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[[File:File:T7 autoloop ctrl-26.jpg|450px|thumb|center|E.coli T7 autoloop without terminator at 37°C  (gfp image)]]
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[[File:T7_loop_1.jpg|450px|thumb|center|E.coli T7 autoloop with terminator  at 37°C  (trans image)]]
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[[File:T7 loop 1 gfp.jpg|450px|thumb|center|E.coli T7 autoloop with terminator at 37°C  (gfp image)]]
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[[File:T7 autoloop ctrl-10.jpg|450px|thumb|center|E.coli negative control  at 37°C  (trans image)]]
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[[File:T7 autoloop ctrl-11.jpg|450px|thumb|center|E.coli negative control at 37°C  (gfp image)]]

Revision as of 03:46, 22 September 2011


E.coli T7 autoloop without terminator at 37°C (trans image)
File:File:T7 autoloop ctrl-26.jpg
E.coli T7 autoloop without terminator at 37°C (gfp image)
E.coli T7 autoloop with terminator at 37°C (trans image)
E.coli T7 autoloop with terminator at 37°C (gfp image)
E.coli negative control at 37°C (trans image)
E.coli negative control at 37°C (gfp image)