Difference between revisions of "Part:BBa I746908:Experience"

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===Applications of BBa_I746908===
 
===Applications of BBa_I746908===
 
<gallery>
 
<gallery>
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[[Image:DTU-Relative_promoter_activity.png|370px|thumb|right|The relative promoter activities of the SPL and the rationally designed promoters. The data is relative to the wildtype I746908, shown in grey]]
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Image:DTU-Relative_promoter_activity.png|The relative promoter activities of the SPL and the rationally designed promoters. The data is relative to the wildtype I746908, shown in grey
 
</gallery>
 
</gallery>
  
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An SPL, and 4 rationally designed mutants were generated based on this part.
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DTU 2011 - This BioBrick was used as template for generating an SPL and 4 rationally designed mutants to expand the dynamic range of the [https://parts.igem.org/wiki/index.php?title=Part:BBa_I0500 I0500] promoter.
  
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The details of generating this can be found [http://2011.igem.org/Team:DTU-Denmark/Project_experiment#Improving_the_araBAD_promoter here]
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The details of generating this can be found [http://2011.igem.org/Team:DTU-Denmark/Project_improving_araBAD here]
  
 
===User Reviews===
 
===User Reviews===
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<I>Lri</I>
 
<I>Lri</I>
 
|width='60%' valign='top'|
 
|width='60%' valign='top'|
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This was used as a reporter for creating an inducible SPL. It worked as expected.
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This was part used as a reporter for creating an inducible SPL. It worked as expected, and produced GFP when induced with arabinose.
  
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[[Image:DTU-Strain_300.GFPvsBiomass_v4.png|370px|thumb|right|The promoter activity of the I746908 Biobrick when induced by 0.2% arabinose. Data originates from a BioLector. The linear regression y = -2.5523 + 0.1095x has an adjusted R-square of 0.974]]
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[[Image:DTU-Strain_300.GFPvsBiomass_v4.png|370px|thumb|right|The promoter activity of the I746908 Biobrick when induced by 0.2% arabinose. Data originates from a BioLektor. The linear regression y = -2.5523 + 0.1095x has an adjusted R-square of 0.974]]
  
 
|};
 
|};
  
 
<!-- DON'T DELETE --><partinfo>BBa_I746908 EndReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_I746908 EndReviews</partinfo>

Latest revision as of 03:40, 22 September 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I746908

  • The relative promoter activities of the SPL and the rationally designed promoters. The data is relative to the wildtype I746908, shown in grey

DTU 2011 - This BioBrick was used as template for generating an SPL and 4 rationally designed mutants to expand the dynamic range of the I0500 promoter.

The details of generating this can be found [http://2011.igem.org/Team:DTU-Denmark/Project_improving_araBAD here]

User Reviews

UNIQb1a6b9ed544bb33e-partinfo-00000001-QINU

•••••

Lri

This was part used as a reporter for creating an inducible SPL. It worked as expected, and produced GFP when induced with arabinose.

The promoter activity of the I746908 Biobrick when induced by 0.2% arabinose. Data originates from a BioLektor. The linear regression y = -2.5523 + 0.1095x has an adjusted R-square of 0.974
;

UNIQb1a6b9ed544bb33e-partinfo-00000003-QINU