Difference between revisions of "Part:BBa K208005:Experience"

Latest revision as of 03:19, 22 September 2011

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Please enter how you used this part and how it worked out.

Applications of BBa_K208005

Done by iGEM11 Potsdam_Bioware

The TorA signal sequence was used in combination with the beta lactamase (BBa_I757010) as a new protease activity detector device. The BioBrick parts BBa_K627012 and BBa_K627013 consist of the TorA signal sequence followed by a short peptide sequence used as cleavage site for different proteases (TEV (BBa_K627008-BBa_K627010) and 14_3C (BBa_K627011)) and ends with the beta lactamase. When E.coli XL1 blue where transformed with our construct, the cells were able to survive at ampicillin concentrations up to 400 µg/ml, when incubated on agar plates over night at 37°C or at 30 °C, which indicates a high export rate via the TAT pathway.

User Reviews

UNIQcee363828e93e55a-partinfo-00000000-QINU UNIQcee363828e93e55a-partinfo-00000001-QINU

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 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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 ===Applications of BBa_K208005===
+Done by iGEM11 Potsdam_Bioware<br><br>
+The TorA signal sequence was used in combination with the beta lactamase (BBa_I757010) as a new protease activity detector device. The BioBrick parts BBa_K627012 and BBa_K627013 consist of the TorA signal sequence followed by a short peptide sequence used as cleavage site for different proteases (TEV (BBa_K627008-BBa_K627010) and 14_3C (BBa_K627011)) and ends with the beta lactamase.
+When E.coli XL1 blue where transformed with our construct, the cells were able to survive at ampicillin concentrations up to 400 µg/ml, when incubated on agar plates over night at 37°C or at 30 °C, which indicates a high export rate via the TAT pathway.
 ===User Reviews===