Difference between revisions of "Part:BBa K208005:Experience"
(→Applications of BBa_K208005) |
(→Applications of BBa_K208005) |
||
| Line 7: | Line 7: | ||
The TorA signal sequence was used in combination with the beta lactamase (BBa_I757010) as a new protease activity detector device. The BioBrick parts BBa_K627012 and BBa_K627013 consist of the TorA signal sequence followed by a short peptide sequence used as cleavage site for different proteases (TEV (BBa_K627008-BBa_K627010) and 14_3C (BBa_K627011)) and ends with the beta lactamase. | The TorA signal sequence was used in combination with the beta lactamase (BBa_I757010) as a new protease activity detector device. The BioBrick parts BBa_K627012 and BBa_K627013 consist of the TorA signal sequence followed by a short peptide sequence used as cleavage site for different proteases (TEV (BBa_K627008-BBa_K627010) and 14_3C (BBa_K627011)) and ends with the beta lactamase. | ||
| − | When E.coli XL1 blue where transformed with our construct, the cells were able to survive at ampicillin concentrations up to 400 µg/ml, when incubated on agar plates over night at 37°C | + | When E.coli XL1 blue where transformed with our construct, the cells were able to survive at ampicillin concentrations up to 400 µg/ml, when incubated on agar plates over night at 37°C or at 30 °C, which indicates a high export rate via the TAT pathway. |
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 03:19, 22 September 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K208005
Done by iGEM11 Potsdam_Bioware
The TorA signal sequence was used in combination with the beta lactamase (BBa_I757010) as a new protease activity detector device. The BioBrick parts BBa_K627012 and BBa_K627013 consist of the TorA signal sequence followed by a short peptide sequence used as cleavage site for different proteases (TEV (BBa_K627008-BBa_K627010) and 14_3C (BBa_K627011)) and ends with the beta lactamase. When E.coli XL1 blue where transformed with our construct, the cells were able to survive at ampicillin concentrations up to 400 µg/ml, when incubated on agar plates over night at 37°C or at 30 °C, which indicates a high export rate via the TAT pathway.
User Reviews
UNIQc1134caf51ee0872-partinfo-00000000-QINU UNIQc1134caf51ee0872-partinfo-00000001-QINU