Difference between revisions of "Part:BBa K525401"

Latest revision as of 03:15, 22 September 2011

S-Layer SbpA from Lysinibacillus sphaericus

S-layer SbpA from Lysinbacillus sphaericus

S-layers (crystalline bacterial surface layer) are crystal-like layers consisting of multiple protein monomers and can be found in various (archae-)bacteria. They constitute the outermost part of the cell wall. Especially their ability for self-assembly into distinct geometries is of scientific interest. At phase boundaries, in solutions and on a variety of surfaces they form different lattice structures. The geometry and arrangement is determined by the C-terminal self assembly-domain, which is specific for each S-layer protein. The most common lattice geometries are oblique, square and hexagonal. By modifying the characteristics of the S-layer through combination with functional groups and protein domains as well as their defined position and orientation to eachother (determined by the S-layer geometry) it is possible to realize various practical applications ([http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.2006.00573.x/full Sleytr et al., 2007]).

Usage and Biology

S-layer proteins can be used as scaffold for nanobiotechnological applications and devices by e.g. fusing the S-layer's self-assembly domain to other functional protein domains. It is possible to coat surfaces and liposomes with S-layers. A big advantage of S-layers: after expressing in E. coli and purification, the nanobiotechnological system is cell-free. This enhances the biological security of a device.

This S-layer gene is in the Freiburg BioBrick Assembly standard so functional protein domains can easily be fused to its C- or N-terminus. SbpA was characterized with BBa_K525405.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 32
Illegal BglII site found at 149
Illegal XhoI site found at 1924
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 4
Illegal AgeI site found at 3121
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 421
Illegal BsaI.rc site found at 550

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K525401 short</partinfo>
-S-Layer sbpA from Lysinibacillus sphaericus
+[[Image:Bielefeld-Germany2011-S-Layer-Geometrien.jpg|300px|thumb|right]]
+S-layer SbpA from ''Lysinbacillus sphaericus''
+S-layers (crystalline bacterial surface layer) are crystal-like layers consisting of multiple protein monomers and can be found in various (archae-)bacteria. They constitute the outermost part of the cell wall. Especially their ability for self-assembly into distinct geometries is of scientific interest. At phase boundaries, in solutions and on a variety of surfaces they form different lattice structures. The geometry and arrangement is determined by the C-terminal self assembly-domain, which is specific for each S-layer protein. The most common lattice geometries are oblique, square and hexagonal. By modifying the characteristics of the S-layer through combination with functional groups and protein domains as well as their defined position and orientation to eachother (determined by the S-layer geometry) it is possible to realize various practical applications ([http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.2006.00573.x/full Sleytr ''et al.'', 2007]).
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+S-layer proteins can be used as scaffold for nanobiotechnological applications and devices by e.g. fusing the S-layer's self-assembly domain to other functional protein domains. It is possible to coat surfaces and liposomes with S-layers. A big advantage of S-layers: after expressing in ''E. coli'' and purification, the nanobiotechnological system is cell-free. This enhances the biological security of a device.
+This S-layer gene is in the Freiburg BioBrick Assembly standard so functional protein domains can easily be fused to its C- or N-terminus. SbpA was characterized with <partinfo>K525405</partinfo>.
 <!-- -->