Difference between revisions of "Part:BBa K628000"

 
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<partinfo>BBa_K628000 short</partinfo>
 
<partinfo>BBa_K628000 short</partinfo>
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This is the coding region for protegrin-1.
 
This is the coding region for protegrin-1.
  
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===Usage and Biology===
 
===Usage and Biology===
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Protegrin-1 coding region under the influence of a pBad-strong promoter. Protegrin-1 is an '18-residue beta-sheet peptide isolated from porcine leukocytes with antimicrobial activity against a broad range of microorganisms.' (Steinberg et al 1997) It has its effect by pore membrane disruption (Lam et al 2006) and possibly also by effects such as activation of membrane-damaging proteases (Bierbaum 1985) and has anti-microbial activity against E. coli (Aumelas 2004).
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K628000 parameters</partinfo>
 
<partinfo>BBa_K628000 parameters</partinfo>
 
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===References===
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Steinberg et al (1997) 'Protegrin-1: a broad-spectrum, rapidly microbicidal peptide with in vivo activity Antimicrobial Agents and Chemotherapy', Aug 1997, 1738-1742, Vol 41, No. 8
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Lam et al (2006) 'Mechanism of Supported Membrane Disruption by Antimicrobial Peptide Protegrin-1' J. Phys. Chem. B, 2006, 110 (42), pp 21282–21286
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Bierbaum, G. & Sahl, H.-G (1985) 'Induction of autolysis of Staphylocci by the basic peptide antibiotics pep5 and nisin and their influence on the activity of autolytic enzymes.' Arch. Microbiol. 141, 249 ± 254
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Aumelas et al. (2004) 'Synthesis and Solution Structure of the Antimicrobial Peptide Protegrin-1' European Journal of Biochemistry Volume 237, Issue 3, pages 575–583, May 1996

Revision as of 03:09, 22 September 2011

codes for protegrin-1 (anti-microbial peptide)

This is the coding region for protegrin-1.


Usage and Biology

Protegrin-1 coding region under the influence of a pBad-strong promoter. Protegrin-1 is an '18-residue beta-sheet peptide isolated from porcine leukocytes with antimicrobial activity against a broad range of microorganisms.' (Steinberg et al 1997) It has its effect by pore membrane disruption (Lam et al 2006) and possibly also by effects such as activation of membrane-damaging proteases (Bierbaum 1985) and has anti-microbial activity against E. coli (Aumelas 2004). Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 8
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Steinberg et al (1997) 'Protegrin-1: a broad-spectrum, rapidly microbicidal peptide with in vivo activity Antimicrobial Agents and Chemotherapy', Aug 1997, 1738-1742, Vol 41, No. 8

Lam et al (2006) 'Mechanism of Supported Membrane Disruption by Antimicrobial Peptide Protegrin-1' J. Phys. Chem. B, 2006, 110 (42), pp 21282–21286

Bierbaum, G. & Sahl, H.-G (1985) 'Induction of autolysis of Staphylocci by the basic peptide antibiotics pep5 and nisin and their influence on the activity of autolytic enzymes.' Arch. Microbiol. 141, 249 ± 254

Aumelas et al. (2004) 'Synthesis and Solution Structure of the Antimicrobial Peptide Protegrin-1' European Journal of Biochemistry Volume 237, Issue 3, pages 575–583, May 1996